微波輔助螢光標定毛細管電泳法對安非他命狡詐家濫用藥物的分離與偵測

Abstract

本研究利用微波輔助螢光標定應用於毛細管電泳分析方法，用來分析鄰、間、對-氯安非他命和鄰、間、對-氟安非他命這六種安非他命濫用藥物與 2,5-dimethoxy- 4-ethylthio-phenethylamine（2C-T-2）、2,5-dimethoxy-4-（n）-propylthiophen- ethylamine（2C-T-7）、4-chloro-2,5- dimethoxyphenethylamine（2C-C）、4-bromo-2,5-dimethoxy-phenethylamine（2C-B）及 2,5-dimethoxy-4-iodo- phenethylamine（2C-I）五種 2C 系列的苯乙胺類毒品。利用 FITC（fluorescein isothiocyanate isomer I）當作螢光標記並使用藍光雷射（473 nm）作激發光源進行偵測。在過去使用 FITC 作衍生劑的衍生時間至少需要 20 小時，現今使用微波輔助衍生所需時間約 5 分鐘即可完成。A microwave-assisted fluorescence labeling method for use in CE-LIF （capillary electrophoresis-laser induced fluorescence） is described. Six amphetamine-like designer drugs, namely, o-, m-, p-chloro- and o-, m-, p-fluoro-amphetamine derivatives, and five 2C-series of phenethylamine designer drugs, including 2,5-dimethoxy-4-ethylthio-phenethylamine （2C-T-2）, 2,5-dimethoxy-4-（n）-propylthiophenethylamine（2C-T-7）, 4-chloro-2,5-dimethoxy-phenethylamine（2C-C）, 4-bromo-2,5-dimethoxy- phenethylamine（2C-B）, 2,5-dimethoxy-4-iodo-phenethylamine（2C-I） derivatives, were synthesized and used as model compounds. FITC （fluoresceinisothiocyanate isomer I） and a blue-laser were used as the fluorescent labeling reagent and excitation source, respectively. When a microwave oven was used, the reaction was complete within ~ 5 min,while the classical method required at least 20 h（usually, an overnight reaction）