以酪氨酸酶、殼聚醣、氧化還原石墨烯製備高靈敏度和選擇性的網印印刷碳電極用於多巴胺檢測
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2018
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多巴胺是人體中重要的神經傳遞物質，其對於帕金森氏症、阿茲海默症皆有重大的影響。在實驗中以酪胺酸酶 (tyrosinase)、殼聚醣 (chitosan)、氧化還原石墨烯 (reduced graphite oxide，rGO) 修飾於網印印刷碳電極 (Screen-printed carbon electrode，SPCE) 作為電化學生物感測器，用於多巴胺的檢測。並且針對抗壞血酸以及尿酸，這類在生物體內常見的干擾物，可以避免此感測器不被干擾而影響偵測誤判。利用循環伏安法測量證明了，所提出的電化學感測器的高靈敏度和選擇性，偵測極限為22 nM，並且與先前文獻相比，有較廣的線性範圍為0.4-8 μM和40-500 μM。此外，所提出的電極被應用於健康人體的尿液樣品時，取得了令人滿意的準確率，表示其適用於生理樣品中多巴胺的分析。
Dopamine (DA) is an important neurotransmitter in human body. It has an great effect on Parkinson's disease and Alzheimer's disease. In this study, we fabricated the electrochemical biosensor of screen-printed carbon electrode (SPCE) modified with tyrosinase, chitosan and redox graphene oxide (rGO) and the sensor was used for the detection of DA without the interference from ascorbic acid or uric acid. We demonstrated the high sensitivity and selectivity of the electrochemical sensors with detection limit of 22 nM, and broad linear ranges of 0.4-8 μM and 40-500 μM by cyclic voltammetry. In addition, the proposed electrode exhibited a satisfactory recovery rate when it applied to healthy human urine samples, indicating that it is suitable for the analysis of DA in physiological samples.
Dopamine (DA) is an important neurotransmitter in human body. It has an great effect on Parkinson's disease and Alzheimer's disease. In this study, we fabricated the electrochemical biosensor of screen-printed carbon electrode (SPCE) modified with tyrosinase, chitosan and redox graphene oxide (rGO) and the sensor was used for the detection of DA without the interference from ascorbic acid or uric acid. We demonstrated the high sensitivity and selectivity of the electrochemical sensors with detection limit of 22 nM, and broad linear ranges of 0.4-8 μM and 40-500 μM by cyclic voltammetry. In addition, the proposed electrode exhibited a satisfactory recovery rate when it applied to healthy human urine samples, indicating that it is suitable for the analysis of DA in physiological samples.
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網印印刷電化學生物感測器, 酪氨酸酶, 殼聚醣, 多巴胺, 氧化還原石墨烯, screen-printed electrochemical biosensor, tyrosinase, chitosan, dopamine, reduced graphene oxide