表面電漿共振原理應用於氣液相化學偵測器之研製
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2013
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Abstract
本研究發展出新型態氣相與液相感測器，其偵測原理是利用奈米金粒子產生的表面電漿共振現象，在中空光纖內層表面修飾3-aminopropyltrimethoxysilane (APTMS)，奈米金粒子自組裝於修飾後的中空光纖內層，將此奈米金粒子中空光纖感測器串聯氣相層析儀，當有機揮發氣體 (VOCs) 經層析分離管柱沖提出來到達奈米金粒子中空光纖感測器，中空光纖內的奈米金粒子吸附有機揮發氣體，造成綠光 (λ=520 nm) 的光吸收度變化。此感測器成功檢測 8 種混合性有機揮發氣體，其結果顯示具有良好的靈敏度、穩定性，其線性關係 ( R2≧0.99 ) 、再現性以及偵測極限範圍 20.0 ~ 180.0 ng，奈米金平板式液相感測器部分，將奈米金粒子自組裝於玻璃平板上，並在其中填滿表面修飾過奈米金粒子的玻璃微粒，將奈米金平板式液相感測器串聯流動注入偵測裝置及液相層析儀，成功的偵測常見醣類。
In this research, we developed two novel devices to be used as gas and liquid chromatographic detectors. The detection mechanism of gas detector was based on the localized surface plasmon resonance of gold nanoparticles that were self-assemble on the inner surface of optical hollow fiber modified with 3-aminopropyltrimethoxysilane (APTMS). A green light emitting diode was used as a light source and the light (=520 nm) transmitted through the glass wall of optical hollow fiber. Once VOCs were eluted from the GC column to the optical hollow fiber, the VOCs were absorbed on the Au-nanoparticles, the light intensity reduced as the absorbance of LSPR band increased. A mixture of eight VOCs was detected by the LSPR-GC detector. The estimated limit of detection was ranged from 20.0 to 180.0 ng and the responses were rapid, reversible, linear (R2≧0.99). The device for liquid chromatographic detector used a micro fluidic channel that assembled between glass substrates. The fluidic channel wasfilled with glass beads that were modified with Au-nanoparticle monolayer. The liquid phase detector was tested on both flow injection system and high performance liquid chromatographic system. The detection signal of several sugar compounds was successfully obtained.
In this research, we developed two novel devices to be used as gas and liquid chromatographic detectors. The detection mechanism of gas detector was based on the localized surface plasmon resonance of gold nanoparticles that were self-assemble on the inner surface of optical hollow fiber modified with 3-aminopropyltrimethoxysilane (APTMS). A green light emitting diode was used as a light source and the light (=520 nm) transmitted through the glass wall of optical hollow fiber. Once VOCs were eluted from the GC column to the optical hollow fiber, the VOCs were absorbed on the Au-nanoparticles, the light intensity reduced as the absorbance of LSPR band increased. A mixture of eight VOCs was detected by the LSPR-GC detector. The estimated limit of detection was ranged from 20.0 to 180.0 ng and the responses were rapid, reversible, linear (R2≧0.99). The device for liquid chromatographic detector used a micro fluidic channel that assembled between glass substrates. The fluidic channel wasfilled with glass beads that were modified with Au-nanoparticle monolayer. The liquid phase detector was tested on both flow injection system and high performance liquid chromatographic system. The detection signal of several sugar compounds was successfully obtained.
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表面電漿共振, 揮發性有機氣體, 奈米金粒子, Localized surface plasmon resonance, Volatile organic compounds, Nanoparticle