前濃縮－多維度氣相層析微晶片系統應用於揮發性有機氣體分析之研究
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2015
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本研究以偵測揮發性有機氣體(VOCs)為主軸，結合前濃縮與多維層析技術，開發出一套分析系統。在採樣及進樣部分使用六向閥自動進樣，利用Carbopack B、Carbopack X及Carboxen 1000三者為吸附劑，製作多階式管狀前濃縮器，利用其吸附劑對分析物進行捕捉，再以加熱脫附的方式提高進入二維層析系統之分析物的濃度。
分離管柱利用機電系統製程技術(micro-electro mechanical systems，MEMS)製作微型微層析晶片。DB-1微型微層析晶片(3 m)做第一維晶片，先對分析物做初步分離，第二維晶片有兩種分析模式，使用四向閥控制樣品流向，分析低沸點化合物時採用碳分子篩微層析晶片(1 m)，做μGC - μGC層析；分析中高沸點化合物時使用多管柱微層析晶片(OV-210、OV-225、OV-330，各1 m)，以三向閥做stop flow二維層析 (μGC × μGC)，利用三靜相的不同選擇性得到三張二維層析圖，可進行交叉比對。利用模擬低濃度進樣系統，分析流道總長度僅4 m，搭配致冷片對微層析晶片進行溫度控制，可以成功的分離沸點 -161℃ ~ 186℃(methane ~ 5-nonanone)的49種含不同官能基的化合物。
This research is based on the detection of volatile organic compounds (VOCs), we combine preconcentrator with multi-dimensional gas chrom- atogramphy technique, and develope an analysis system. A six-way valve is used to sampling and injecting analytes. Carbopack B,Carbopack X and Carboxen 1000 are used as the adsorbent of the muti-stage precon- centrator, it can concentrate analytes before thermal desorption into two- dimensional gas chromatography system to increase the injection mass of analyte. The μ-column chips are fabricated using micro-electro mechanical systems(MEMS) process. We use DB-1 μ-column (3 m) as the first column, it performs preliminary separation for analytes, then the gas flow is divide into two different parts by a four-way valve. When analyzing low boiling point compounds, carbon molecular sieve are used to do μGC - μGC analysis; moderate and high boiling point compounds, we apply three parallel μ-columns chip(OV-210, OV-225 and OV-330, 1 m each) and three-way valve to do μGC × μGC comprehensive two- dimensional gas chromatography. Due to the different selectivity of three stationary phase, we can get three different two dimensional chromatograms in this part. In this study, we simulate low concentration injection, using thermoelectric cooling modulate to control the temperature of three μ-column chips. We successfully separate 49 compounds with boiling point ranged from -161℃ to 186℃(methane ~ 5-nonanone).
This research is based on the detection of volatile organic compounds (VOCs), we combine preconcentrator with multi-dimensional gas chrom- atogramphy technique, and develope an analysis system. A six-way valve is used to sampling and injecting analytes. Carbopack B,Carbopack X and Carboxen 1000 are used as the adsorbent of the muti-stage precon- centrator, it can concentrate analytes before thermal desorption into two- dimensional gas chromatography system to increase the injection mass of analyte. The μ-column chips are fabricated using micro-electro mechanical systems(MEMS) process. We use DB-1 μ-column (3 m) as the first column, it performs preliminary separation for analytes, then the gas flow is divide into two different parts by a four-way valve. When analyzing low boiling point compounds, carbon molecular sieve are used to do μGC - μGC analysis; moderate and high boiling point compounds, we apply three parallel μ-columns chip(OV-210, OV-225 and OV-330, 1 m each) and three-way valve to do μGC × μGC comprehensive two- dimensional gas chromatography. Due to the different selectivity of three stationary phase, we can get three different two dimensional chromatograms in this part. In this study, we simulate low concentration injection, using thermoelectric cooling modulate to control the temperature of three μ-column chips. We successfully separate 49 compounds with boiling point ranged from -161℃ to 186℃(methane ~ 5-nonanone).
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前濃縮, 多階式吸附劑, 微機電系統製程, 二維氣相層析, preconcentrator, muti-stage adsorbent, MEMS, 2D-GC