紫外光發光二極體誘導螢光偵測法結合毛細管電泳/線上濃縮技術之開發與應用

dc.contributor林震煌zh_TW
dc.contributor.author張喦升zh_TW
dc.date.accessioned2019-09-04T09:04:34Z
dc.date.available2007-6-30
dc.date.available2019-09-04T09:04:34Z
dc.date.issued2005
dc.description.abstract本篇實驗應用紫外光發光二極體(UV-LED)誘導螢光搭配毛細管電泳層析技術,並結合線上濃縮技術對於螢光性(核黃素)及非螢光性(色氨酸)進行偵測。本實驗所使用的UV-LED發光波長在380 nm,功率約在2 mW。在偵測核黃素的部分使用速度變化誘導聚焦(Velocity-Difference Induced Focusing, V-DIF)來進行實驗,成功將偵測極限由原來僅使用微胞電動電泳層析的200ppb提高到3~7ppb。在色氨酸的偵測部分,則利用螢光試劑衍生色氨酸,使其適合UV-LED的激發,並利用掃集技術(sweeping)將偵測極限從原來使用微胞電動電泳層析的1.5ppm提高至3ppb。在真實樣品的應用上分別偵測啤酒裡面的核黃素,以及尿液、牛奶裡面的色氨酸均可以成功快速達到定性及定量的結果。zh_TW
dc.description.abstractAbstract The application of an ultraviolet (UV) light emitting diode (LED) to on-line sample concentration/fluorescence detection in capillary electrophoresis (CE) is described. The utility of UV-LED (peak emission wavelength at 380 nm, ~ 2 mW) for fluorescence detection is demonstrated by examining a naturally fluorescent (riboflavin) and a non-fluorescent compound (tryptophan), respectively. The detection limit for riboflavin was determined to be 0.2 ppm by the normal MEKC mode and this was improved to 3 ~ 7 ppb when a dynamic pH-junction techniques were applied. On the other hand, the detection limit of the tryptophan derivative was determined to be 1.5 ppm using the MEKC mode and this was improved to 3 ppb when the sweeping-MEKC mode was applied. In an analysis of an actual sample, the concentrations of riboflavin and tryptophan in beer and urine/milk samples were determined, respectively.en_US
dc.description.sponsorship化學系zh_TW
dc.identifierG0069142001
dc.identifier.urihttp://etds.lib.ntnu.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=id=%22G0069142001%22.&%22.id.&
dc.identifier.urihttp://rportal.lib.ntnu.edu.tw:80/handle/20.500.12235/99874
dc.language中文
dc.subject毛細管電泳zh_TW
dc.subject發光二極體zh_TW
dc.title紫外光發光二極體誘導螢光偵測法結合毛細管電泳/線上濃縮技術之開發與應用zh_TW

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