利用偶氮染料做為次氯酸鈉指示劑的開發與研究

Abstract

在本研究中欲探討偶氮染料作為次氯酸鈉的指示劑，使得次氯酸鈉在可見光範圍內被偵測到，並且能夠增加其定量範圍，使其能夠應用在醫療消毒的檢測。利用七種不同的偶氮染料進行篩選，透過紫外可見光譜儀以及手持式的可見光光譜儀，來檢測各個染料對次氯酸鈉的定量範圍，以及在紅綠藍光模式下得到最佳的定量線性範圍。接著檢測各個染料對次氯酸鈉的反應時間，反應時間越短代表越適合作為指示劑。最後再加熱各個染料溶液於不同溫度，來檢測偶氮料的熱穩定性。透過上述實驗的篩選，篩選出酸性橙7最適合做為次氯酸鈉的指示劑。針對酸性橙7做延伸研究，包含將固態酸性橙7附著在樣品槽壁上，方便未來作為商品化的應用。再來檢測次氯酸鈉與酸性橙7的pH值，確保兩者混和後pH值不會有太大的變化。接著利用化學動力學的計算，來佐證利用酸性橙7與次氯酸鈉的定量是可信的。以及利用液相層析電噴灑質譜儀，比較酸性橙7溶液與和次氯酸鈉反應後的溶液，來推測此反應的反應機制。In this study, azo dyes were investigated as indicators of sodium hypochlorite to detect sodium hypochlorite in the visible range and to increase its linear dynamic range for medical disinfection applications.Seven different azo dyes were screened by UV-visible spectrometer and handheld visible spectrometer to detect the quantitative range of each dye for sodium hypochlorite and to obtain the best linear dynamic range in red, green and blue light mode. Then, the reaction time of each dye to sodium hypochlorite was examined. The shorter the reaction time, the more suitable as an indicator. Finally, the thermal stability of the azo materials was examined by heating each dye solution at different temperatures. Through the above screening, Acid Orange 7 was selected as the most suitable indicator for sodium hypochlorite.Next, extension studies were conducted on Acid Orange 7, which included coating the solid Acid Orange 7 to the sample tank wall for future commercialization. The pH value of sodium hypochlorite and acid orange 7 was checked to ensure that the pH value would not be influence too much after mixing the two substances. Then, chemical kinetics calculations were used to confirm the reliability of the quantification using acid orange 7 and sodium hypochlorite. The reaction mechanism of the reaction was inferred by comparing the solution of acid orange 7 with the solution of sodium hypochlorite using liquid phase chromatography electrospray ionization mass spectrometry.