氣相層析/液相層析/毛細管電泳法對九種色胺類化合物的分離與分析

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2008

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本實驗以氣相層析、液相層析及毛細管電泳三種分離方法進行研究,實驗分析物為九種色胺類衍生物 (α-methyltryptamine, AMT; N,N-dimethyltryptamine, DMT;5-methoxy-α-methyltryptamine, 5-MeO-AMT;N,N-diethyltryptamine, DET; N,N-dipropyltryptamine, DPT;N,N-dibutyltryptamine, DBT;N,N-diisopropyltryptamine, DIPT; 5-methoxy-N,N-dimethyltryptamine, 5-MeO-DMT; 5-methoxy-N,N-diisopropyltryptamine, 5-MeO-DIPT)。這九種分析物被選作測試樣品,是爲了用來比較它們相對的偵測靈敏度、選擇性、分離所需時間、分離所需成本與分離時先後出現的順序等。 實驗結果發現,當S/N = 3時,氣相層析質譜法與液相層析/紫外光吸收法得到的偵測極限分別為0.5 ~ 15與0.3 ~1 μg/mL。相對於這兩種方法,使用毛細管區帶電泳/紫外光吸收法時,偵測極限可改善至0.4 ~ 1 μg/mL。當引用線上掃集-微胞電動的技術時,偵測極限甚至於還可大幅改善至2 ~ 8 ng/mL。 至於分離九種色胺類衍生物所需的時間,使用氣相層析法時,分離時間為11 ~ 15分鐘;使用液相層析法時,分離時間為8 ~ 23分鐘;使用線上掃集-微胞電動技術則需要20 ~ 26分鐘。分析物出現的先後順序基本上依據其分子量的大小,例如DMT最先出現,然後才是DET和DPT,最後才是DBT。但是,當分析物為一級胺 (例如AMT與5-MeO-AMT )、同分異構物 (DIPT與DPT) 或有5-甲醚基的官能基(例如5-MeO-AMT、5-MeO-DMT或 5-MeO-DIPT) 時,分離條件改變時,順序也會略有變動。
Nine tryptamines, including α-methyltryptamine (AMT), N,N-dimethyltryptamine (DMT), 5-methoxy-α-methyltryptamine (5-MeO-AMT), N,N-diethyltryptamine (DET), N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT), N,N-diisopropyltryptamine (DIPT), 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT), and 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) were selected as model compounds. Comparisons of their sensitivity, selectivity, time, cost and the order of migration are described based on different separation techniques (GC,HPLC and CE, respectively). As a result, the limit of detection (S/N = 3) obtained by GC/MS and LC/UV-absorption ranged from 0.5 to 15 μg/mL and 0.3 to 1.0 μg/mL, respectively. In contrast to this, based on the CZE/UV-absorption method, the limit of detection (S/N = 3) was determined to 0.4–1 μg/mL. However, when the sweeping-MEKC mode was applied, it dramatically improved to 2–8 ng/mL. In the case of GC, HPLC and CE, migration times of the nine standards ranged from 11 to 15 min and 8 to 23 min by GC and HPLC, respectively; ranged from 20 to 26 min by sweeping-MEKC. The order of migration of DMT, DET, DPT and DBT follows the molecular weight, whereas the order of migration of AMT and 5-MeO-AMT (primary amines), DIPT (an isomer of DPT) and 5-methoxy-tryptamines (5-MeO-AMT, 5-MeO-DMT and 5-MeO-DIPT) can be altered by changing the separation conditions.

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色胺類, tryptamine

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