利用紙片電噴灑質譜快速篩選及毛細管電泳質譜測定唾液中含4-氯安非他命的開發與研究

Abstract

本實驗開發了一種新型藥物篩選系統，包括濾紙電噴霧質譜（PS-MS）和毛細管電泳電噴灑質譜法（CE-ESI-MS）的方法。本實驗系統可以很容易地切換的PS-MS快速篩選樣品或傳統CE-ESI-MS分離方法，並利用同時共享同一台質譜而獲得詳細的質譜信息。在PS-MS情況下，當用尖端為（15度）的濾紙時，從紙尖到質譜入口的最佳距離大約口為7.7 mm，而最佳化CE-ESI尖端的距離為13.5 mm。另外本實驗是使用4 -氯安非他命作為測試樣品，而PS-MS和CE-ESI-MS偵測極限經過測試後確定分別為0.1和0.25 ppm。以下是使用PS-MS和CE-ESI-MS和實驗條件下得到的結果的比較與說明。

A novel drug-screening system, consisting of paper spray-mass spectrometry (PS-MS) and a capillary electrophoresis electrospray ionization-mass spectrometry (CE-ESI-MS) method was developed. This system can be easily switched either to PS-MS for rapidly screening samples or to the traditional CE-ESI-MS method for separation and to obtain detailed mass spectral information, while sharing the same mass spectrometer. In the former case, when a sharp (15º-tip) chromatography paper was used, the optimized distance from the paper tip to the mass inlet was 7.7 mm, whereas the optimized distance for the CE-ESI tip was ~ 13.5 mm. Using 4-chloro-amphetamine as a model compound, the limits of detection for PS-MS and CE-ESI-MS were determined to ~ 0.1 and 0.25 ppm, respectively. Comparisons of results obtained using PS-MS and CE-ESI-MS and the experimental conditions are described.