利用螢光二氧化矽奈米管作為DNA雜交之生化反應器及其分析

Abstract

本文主要是以螢光二氧化矽奈米管作為模擬DNA晶片來檢測疾病。其中，所選擇的硬式模板(AAO)擁有固定形狀及孔徑大小之優勢，因此可利用多孔性孔洞之陽極氧化鋁(AAO)和二氧化矽的溶膠-凝膠(sol-gel)溶液，來合成二氧化矽奈米管(SiNTs)，且二氧化矽奈米管本身具有容易修飾及特殊的中空結構，所以可以在內、外部做些修飾來固定生物分子或分離物質的反應。在此論文中，我們藉由所加入具螢光性質的水溶性Ⅱ-Ⅵ半導體奈米粒子CdSe(ZnS)於管內，使得到螢光二氧化矽奈米管後，再與標的有機染料DNA作雜交反應，來獲得有螢光混色效果的二氧化矽奈米管。最後，在我們實驗的結果中，發現所合成的二氧化矽奈米管，可利用短小化特點以較低濃度DNA樣品來達到作為高靈敏度探針之目的。

In this paper, we utilized silica nanotubes mimicing biochips to detect diseases. Silica nanotubes were prepared by a sol-gel reaction using the anodic aluminum oxide membrane (AAO) as a template. By utilizing template methods, a large number of hollow structure nanotubes were synthesized and their sizes were controlled precisely. The silica nanotubes have a number of advantages that make them potential candidates for biological applications. They have inner voids that can be immobilized with biomolecules. Also, they have distinct inner and outer surfaces that can be functionalized differentially. Hence, nanotubes can be used as smart nanophase extractors. In our experiment, the fluorescent silica nanotubes were synthesized by dopping water-soluble nanocrystal CdSe(ZnS). They were then coupled to fluorescent DNA to observe fluorescence change. Finally, we found that our nanotubes have high sensitivity in biochip analysis. So, we combined QDs-tagged silica nanotubes with fluorescent DNA as analytical tools to detect cancer disease.