微型氣相層析儀應用於呼氣丙酮及中草藥材氣味分析之研究

Abstract

糖尿病是 全世界 近幾年來非常盛行的慢性文明病，目前也有學者正研究人體呼出的有機揮發性氣 體 (VOCs)所帶給人體生理上的反應，以及利用非侵入式的方法檢測人體 。 另外， 近年來中草藥材中所散發出的氣味 是否正確 也成為人們生活上的一項課題，相同的中草 藥材卻散發出不同的 香氣 ，目前有許多研究與電子鼻相關，模擬了人體的嗅覺模式去進行不同物種的比對。本論文主要以人體呼出 的 有機揮發物以及中草藥材 氣味化合物這兩項為研究主題，人體呼氣的受試者招募了 共 12位 糖尿病患者以及 生理健康人體， 利用本實驗室自製的 氣體採集袋 進行呼氣檢測，而中藥材氣味找尋了杜仲的傳統中草藥材和科學中藥，模擬頂空 的方式採集中草藥材上方的氣體進行研究利用氣相層析質譜儀和本實驗室開發的微型氣相層析儀 進行分析 。本論文研究出了糖尿病患者與呼氣丙酮濃度的相關，以及人體呼氣出的有機揮發物 反應了人體一定的代謝狀態，也探討相同中草藥材於不同產地採收所散發出氣味化合物的異同，和複方科學中藥透過單方科學中藥混合時，所散發出的氣味和濃度是否有加成性 ，並以微型氣相層析儀取代電子鼻，使數據有更高的精確度。Diabetes is a chronic disease that is very prevalent all over the world in recent years. At present, some scholars are studying the physiological response of the human body's exhaled Organic Volatile Compounds (VOCs), and the use of non-invasive methods to detect the human body's breath. In addition, in recent years, whether the smell emitted by herbal medicine is correct has also become a topic in our life. The same herbal medicine emits different aromas. At present, there are many studies related to Electronic-noses that simulate the human body’s olfactory pattern to perform different scents and comparison of species. The thesis mainly focuses on the two research topics of human body exhaled Organic Volatiles Compounds and herbal medicine compounds. The human exhalation subjects recruited a total of 12 diabetic patients and physically healthy human bodies, and used the self-made gas collection bag in our laboratory to exhale. Detection the smell of herbal medicine searched for herbal medicine materials and scientific herbal medicine of Eucommia ulmoides. The gas above the herbal medicine materials was collected by Headspace (HS). The gas chromatography mass spectrometry and the micro-gas chromatography developed in our laboratory were used for analysis.