利用近紅外雷射光來區域性遙控金奈米棒作為載體的基因表現
Abstract
將材料特性與奈米技術結合，發展出新的生物傳輸系統之奈米載體，預期在未來不僅只是攜帶生物分子，而且若是能夠區域性控制生物分子的釋放，將是未來藥物傳輸之一大進展。在本實驗中我們選用金奈米棒作為生物傳輸系統上之載體，由於金奈米棒具有表面易修飾硫基與對細胞低毒性的特性，再加上它吸收了足夠的雷射光能量時，會產生表面原子重排進而形成較穩定的球狀結構。在實驗中我們利用金奈米棒來傳送一端有修飾硫基的DNA進入細胞中，當近紅外雷射光給了金奈米棒足夠能量時，預期在金奈米棒形變成較穩定的球狀結構時，會在只有近紅外雷射光照射區域才會釋放DNA，而未來更能應用於生物體內目標組織的釋放。
The precious control of the biomolecule releasing on specific localizations of the cell is the key issue for further bio-medical application of bio-functionalized nanomaterials. Herein, we demonstrate that DNA functionalized gold nanorods can be used as a gene carrier. Under the infrared radiation, the structural transformation of gold nanorods into spherical gold nanoparticles results in the realses of DNA in the cells. The cells with the radiation generate the gene expression locally. Our results suggest that the new gene activated technigue using DNA functionalized gold nanorods as a carrier is applicable for gene therapy for specific cells.
The precious control of the biomolecule releasing on specific localizations of the cell is the key issue for further bio-medical application of bio-functionalized nanomaterials. Herein, we demonstrate that DNA functionalized gold nanorods can be used as a gene carrier. Under the infrared radiation, the structural transformation of gold nanorods into spherical gold nanoparticles results in the realses of DNA in the cells. The cells with the radiation generate the gene expression locally. Our results suggest that the new gene activated technigue using DNA functionalized gold nanorods as a carrier is applicable for gene therapy for specific cells.
Description
Keywords
金奈米棒, 基因傳輸, 區域性遙控