毛細管微胞電動層析法-比較陰離子及陽離子界面活性劑對類固醇分析的影響之研究

Abstract

為了增進類固醇分離的選擇性與靈敏度，並且討論分析物在電泳時產生不同的遷移速率，本實驗比較使用不同陰離子及陽離子界面活性劑在毛細管微胞電動層析法中對類固醇分離的影響。利用毛細管電泳/紫外光吸收偵測法分離且偵測到六個混合的類固醇-progesterone、17-hydroxy progesterone、11-deoxycortisol、corticosterone、cortisol、cortisone。類固醇的電泳遷移速率受到背景溶液的酸鹼值、界面活性劑的種類以及分析電壓為正電或負電所影響，這些因素會影響到EOF的大小及方向，或使微胞的遷移速率以及遷移方向發生改變，本實驗將對影響類固醇在電泳時遷移速率的主要因素作討論及整理。運用毛細管電泳掃集技術已大為降低對類固醇的偵測極限，且成功在老鼠及人體血液及尿液樣品中偵測到類固醇的含量。

In attempts to improve the selectivity and sensitivity of steroid separation and to determine their migration order, a comparison of the use of anionic and cationic surfactants based on the MEKC and sweeping-MEKC modes was made. A mixture of six steroids (progesterone, 17-hydroxy progesterone, 11-deoxycortisol, corticosterone,cortisone, and cortisol) could be separated and detected by means of the CE/UV-absorption method. The order of migration time for these steroids was compared under various conditions, including acidic/alkaline buffers, anionic/cationic surfactants, and positive/negative applied voltage, causing the direction of the EOF and the migration of micelles to change. The major rules for generally predicting the migration order of steroids are summarized. The detection limits were significantly improved when the sweeping-MEKC mode was applied. The six steroids were also determined in samples of female urine and blood. The extraction procedures used for the real samples and the CE conditions for their separation were all optimized and the data are reported herein.