Please use this identifier to cite or link to this item: http://rportal.lib.ntnu.edu.tw:80/handle/20.500.12235/80355
Title: A single sequence context cannot satisfy all non-AUG initiator codons in yeast†
Authors: Chang, Chia-Pei
Chen, Shun-Jia
Lin, Chen-Huan
Wang, Tzu-Ling
Wang, Chien-Chia
Issue Date: 9-Jul-2010
Citation: BMC Microbiology. 2010 Jul 09;10(1):188
Abstract: Abstract Background Previous studies in Saccharomyces cerevisiae showed that ALA1 (encoding alanyl-tRNA synthetase) and GRS1 (encoding glycyl-tRNA synthetase) respectively use ACG and TTG as their alternative translation initiator codons. To explore if any other non-ATG triplets can act as initiator codons in yeast, ALA1 was used as a reporter for screening. Results We show herein that except for AAG and AGG, all triplets that differ from ATG by a single nucleotide were able to serve as initiator codons in ALA1. Among these initiator codons, TTG, CTG, ACG, and ATT had ~50% initiating activities relative to that of ATG, while GTG, ATA, and ATC had ~20% initiating activities relative to that of ATG. Unexpectedly, these non-AUG initiator codons exhibited different preferences toward various sequence contexts. In particular, GTG was one of the most efficient non-ATG initiator codons, while ATA was essentially inactive in the context of GRS1. Conclusion This finding indicates that a sequence context that is favorable for a given non-ATG initiator codon might not be as favorable for another.
URI: http://dx.doi.org/10.1186/1471-2180-10-188
http://rportal.lib.ntnu.edu.tw/handle/20.500.12235/80355
ISSN: 1471-2180
Appears in Collections:BMC Springer Open Data



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