A single sequence context cannot satisfy all non-AUG initiator codons in yeast†

dc.contributor.authorChang, Chia-Pei
dc.contributor.authorChen, Shun-Jia
dc.contributor.authorLin, Chen-Huan
dc.contributor.authorWang, Tzu-Ling
dc.contributor.authorWang, Chien-Chia
dc.date.accessioned2018-02-23T17:31:28Z
dc.date.available2018-02-23T17:31:28Z
dc.date.issued2010-07-09
dc.date.updated2018-02-23T17:31:28Z
dc.description.abstractAbstract Background Previous studies in Saccharomyces cerevisiae showed that ALA1 (encoding alanyl-tRNA synthetase) and GRS1 (encoding glycyl-tRNA synthetase) respectively use ACG and TTG as their alternative translation initiator codons. To explore if any other non-ATG triplets can act as initiator codons in yeast, ALA1 was used as a reporter for screening. Results We show herein that except for AAG and AGG, all triplets that differ from ATG by a single nucleotide were able to serve as initiator codons in ALA1. Among these initiator codons, TTG, CTG, ACG, and ATT had ~50% initiating activities relative to that of ATG, while GTG, ATA, and ATC had ~20% initiating activities relative to that of ATG. Unexpectedly, these non-AUG initiator codons exhibited different preferences toward various sequence contexts. In particular, GTG was one of the most efficient non-ATG initiator codons, while ATA was essentially inactive in the context of GRS1. Conclusion This finding indicates that a sequence context that is favorable for a given non-ATG initiator codon might not be as favorable for another.
dc.identifier.citationBMC Microbiology. 2010 Jul 09;10(1):188
dc.identifier.issn1471-2180en_US
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-10-188
dc.identifier.urihttp://rportal.lib.ntnu.edu.tw/handle/20.500.12235/80355
dc.language.rfc3066en
dc.rights.holderChang et al; licensee BioMed Central Ltd.
dc.titleA single sequence context cannot satisfy all non-AUG initiator codons in yeast†
dc.typeJournal Article

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