牛樟芝子實體對四氯化碳誘導慢性肝損傷之功效
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2021
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肝臟是人體最重要的代謝器官，其功能主要包含代謝宿主及外來分子等。由氧化壓力所引起之肝損傷是造成不同肝臟疾病最主要原因之一。四氯化碳(CCl4)是一種肝毒性化合物，常被使用於建立肝損傷之動物模型。牛樟芝(Antrodia cinnamomea, AC)為一種生長於台灣山區之特有種，先前實驗證實其具有解毒、保肝及抗腫瘤等相關活性作用。利用抗氧化物輔助營養或食品補充以減低ROS的產生會是減低長期肝發炎或是慢性肝發炎的策略之ㄧ。故本研究主要是以四氯化碳(CCl4)誘導大白鼠慢性肝損傷，探討不同牛樟芝子實體試驗樣品對於大白鼠慢性肝損傷之影響。研究成果顯示CCl4會造成肝臟組織外觀及病理組織破壞，肝損傷之大鼠其體重上升變化量變大， GOT、GPT、T-cholesterol、ALP、γ-GT表現量會上升，肝臟會產生纖維化，這和4HNE/Caspase-3/PARP介導的細胞凋亡有關，而在Silymarin治療組或牛樟芝子實體試驗樣品組皆可有效緩解CCl4引起的這些效應。這些結果表明，牛樟芝子實體可改善四氯化碳誘導的大鼠肝損傷，未來在輔助食品之應用上具有改善潛力。
The liver is the most important metabolic organ of the human body, and it’s functions mainly include the metabolic host and foreign molecules. Liver injury caused by oxidative stress is one of the most important causes of different liver diseases. Carbon tetrachloride(CCl4) is a hepatotoxic compound that is often used to establish animal models of liver injury. Antrodia cinnamomea is an endemic species that grows in the mountains of Taiwan. Previous experiments have confirmed that it has detoxification, liver protection and anti-tumor activities. Using antioxidants to assist nutrition or food supplements to reduce the production of ROS will be one of the strategies to reduce long-term liver inflammation or chronic liver inflammation. Therefore, this study aim to explored the effects of different Antrodia camphorata fruit bodies test samples on chronic liver injury in rats. Research results show that CCl4 can cause liver tissue appearance and pathological tissue damage. Rats with liver injury have larger changes in weight gain, GOT, GPT, T-cholesterol, ALP, and γ-GT expression levels will increase, and liver fibrosis will occur. This is related to 4HNE/Caspase-3/PARP-mediated apoptosis, which can effectively alleviate these effects caused by CCl4 in the Silymarin treatment group or the Antrodia camphorata fruit body test sample group. These results indicate that Antrodia cinnamomea fruitbodies can improve liver damage induced by carbon tetrachloride in rats, and have potential for improvement in the application of supplementary food in the future.
The liver is the most important metabolic organ of the human body, and it’s functions mainly include the metabolic host and foreign molecules. Liver injury caused by oxidative stress is one of the most important causes of different liver diseases. Carbon tetrachloride(CCl4) is a hepatotoxic compound that is often used to establish animal models of liver injury. Antrodia cinnamomea is an endemic species that grows in the mountains of Taiwan. Previous experiments have confirmed that it has detoxification, liver protection and anti-tumor activities. Using antioxidants to assist nutrition or food supplements to reduce the production of ROS will be one of the strategies to reduce long-term liver inflammation or chronic liver inflammation. Therefore, this study aim to explored the effects of different Antrodia camphorata fruit bodies test samples on chronic liver injury in rats. Research results show that CCl4 can cause liver tissue appearance and pathological tissue damage. Rats with liver injury have larger changes in weight gain, GOT, GPT, T-cholesterol, ALP, and γ-GT expression levels will increase, and liver fibrosis will occur. This is related to 4HNE/Caspase-3/PARP-mediated apoptosis, which can effectively alleviate these effects caused by CCl4 in the Silymarin treatment group or the Antrodia camphorata fruit body test sample group. These results indicate that Antrodia cinnamomea fruitbodies can improve liver damage induced by carbon tetrachloride in rats, and have potential for improvement in the application of supplementary food in the future.
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肝損傷, 氧化壓力, 牛樟芝子實體, 四氯化碳, liver injury, oxidative stress, Antrodia cinnamomea fruiting bodies, carbon tetrachloride