以Figaro二氧化碳感測器監測新舊榕樹葉在不同溫度下之呼吸速率
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2019
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以往計算植物呼吸速率的方式為採用氣相層析質譜儀或使用外界的氣體分析儀採集封閉樣品內的氣體進行氧氣的消耗速率以及二氧化碳生成速率的計算。其過程不僅樣品製作繁瑣且有諸多限制，體積的限制下讓氣相層析質譜儀無法隨身攜帶，只能將植物的葉片摘取回實驗室進行測量。且質譜儀的價格要價不斐，其維護成本也不小，諸多原因讓對植物呼吸速率的研究造成阻礙。再者使用氣體分析儀的情況下，大量抽取封閉系統內的氣體更是會影響呼吸作用。
本研究利用Figaro非分散性紅外線吸收氣體感測器晶片自組裝二氧化碳感測裝置，成功在短時間內觀察到榕樹樹葉於常溫到高溫的範圍下的暗呼吸作用速率。且發現，榕樹的樹葉在新生階段因為需要大量的養分來供給發育所需的能量，所以在常溫階段下(攝氏25度~45度)的呼吸速率會比早已成熟的深綠色老葉快。但到了高溫階段(攝氏45度~70度)，新葉不僅呼吸速率較老葉低，也比老葉更早死亡。推測其原因為新葉的厚度較薄，其角質層也較為不完善，內部的呼吸作用相關酵素無法有效受到保護，所以對於高溫的耐受度相對老葉較為低。
並且本研究開發的感測裝置長24公分、高12公分、寬15公分，且重量僅1.5公斤。方便攜帶的特性只要配合筆記型電腦即可隨地進行植物的呼吸作用速率測量。並且將感測器設計在封閉系統內部減少對呼吸作用造成的影響。
The traditional way to calculate plant respiration rate was to calculate the rate of oxygen consumption or the rate of carbon dioxide generation using a gas chromatography mass spectrometer or a gas analyzer. However, many reasons hinder the research on the respiration rate of plants. With a big of amount volume, the gas chromatography mass spectrometer can’t be carried around, and only the leaves of the plants can be taken back to the laboratory for measurement. In the case of a gas analyzer, a large amount of gas extracted from the closed system will affect the respiration. In this study, a carbon dioxide sensing device was built using a commerciallyavailable NIPD gas sensor which was made by Figaro and successfully used to observe the dark respiration rate of Marabutan leaves in the range from normal temperature to high temperature in a short time. It is also found that the Marburatan leaves which are in the born stage have faster respiration rate than the already mature dark green leaves at normal temperature stage (25 degrees to 35 degrees Celsius) because they need energy for development. However, at high temperature stage (35 degrees to 70 degrees Celsius), the new leaves not only have a lower respiratory rate, but also die earlier than the old leaves. It is speculated that the reason is that the thickness of the new leaves is thin, the stratum corneum is also imperfect, and the inner mitochondria cannot be effectively protected, so the tolerance to high temperature is lower than that of the old leaves. Moreover, the sensing device developed in this study is 24 cm long, 12 cm high, 15 cm wide, and weighs only 1.5 kg. Easy-to-carry features can be used to measure the rate of respiration of plants with a laptop. And the sensor is designed inside the closed system to reduce the impact on leaves respiration.
The traditional way to calculate plant respiration rate was to calculate the rate of oxygen consumption or the rate of carbon dioxide generation using a gas chromatography mass spectrometer or a gas analyzer. However, many reasons hinder the research on the respiration rate of plants. With a big of amount volume, the gas chromatography mass spectrometer can’t be carried around, and only the leaves of the plants can be taken back to the laboratory for measurement. In the case of a gas analyzer, a large amount of gas extracted from the closed system will affect the respiration. In this study, a carbon dioxide sensing device was built using a commerciallyavailable NIPD gas sensor which was made by Figaro and successfully used to observe the dark respiration rate of Marabutan leaves in the range from normal temperature to high temperature in a short time. It is also found that the Marburatan leaves which are in the born stage have faster respiration rate than the already mature dark green leaves at normal temperature stage (25 degrees to 35 degrees Celsius) because they need energy for development. However, at high temperature stage (35 degrees to 70 degrees Celsius), the new leaves not only have a lower respiratory rate, but also die earlier than the old leaves. It is speculated that the reason is that the thickness of the new leaves is thin, the stratum corneum is also imperfect, and the inner mitochondria cannot be effectively protected, so the tolerance to high temperature is lower than that of the old leaves. Moreover, the sensing device developed in this study is 24 cm long, 12 cm high, 15 cm wide, and weighs only 1.5 kg. Easy-to-carry features can be used to measure the rate of respiration of plants with a laptop. And the sensor is designed inside the closed system to reduce the impact on leaves respiration.
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呼吸速率, 非分散性紅外線吸收氣體感測器, 自組裝二氧化碳感測裝置, respiration rate, NDIR gas sensor, self-assembled carbon dioxide sensing device