教師著作
Permanent URI for this collectionhttp://rportal.lib.ntnu.edu.tw/handle/20.500.12235/37076
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Item Photoacoustic Flow Measurements with Gold Nanoparticles(Institute of Electrical and Electronics Engineers (IEEE), 2006-10-01) C.-W. Wei; C.-K. Liao; H.-C. Tseng; Y.-P. Lin; Chia-Chun Chen; P.-C. LiThe hypothesis that quantitative blood flow measurements are feasible with the time-intensity based method in photoacoustic imaging using gold nanoparticles as contrast agent is experimentally tested. The in vitro results show good linearity between the measurements and the theory, thus suggesting the potential of relative photoacoustic flow measurements with gold nanoparticlesItem Applications of Carbohydrate-Gold Nanoparticles for Volumetric Flow Measurements Using an Opto-Acoustic Technique(Taipei, Taiwan, R.O.C. ： The Chinese Chemical Society, 2008-02-01) C.-Y. Yang; C.-W.Wei; Y.-P. Lin; H.-C. Tseng; Y.-N. Wu; Chia-Chun Chen; D.-B. Shieh; P.-C. LiGold nanoparticles with carbohydrate ligands attached on their surface have been synthesized and characterized with various techniques. The new nanoparticle conjugates have shown great potentials as a contrast agent for opto-acoustic imaging. Hemocompatibility measurements of human blood for the carbohydrate-gold nanoparticles have shown that the conjugates are feasible for in vivo testing. Preliminary quantitative flow measurements using the conjugates were also studied in this work based on the indicator-dilution theory. In vitro phantom experiments were designed and conducted, and results were discussed.Item Quantitative Relations of Acoustic Inertial Cavitation with Sonoporation and Cell Viability(Elsevier, 2006-12-01) C.-Y. Lai; C.-H. Wu; Chia-Chun Chen; P.-C. LiUltrasound-induced acoustic cavitation assists gene delivery, possibly by increasing the permeability of the cell membranes. How the cavitation dose is related to the sonoporation rate and the cell viability is still unknown and so this in vitro study quantitatively investigated the effects of cavitation induced by 1-MHz pulsed ultrasound waves and the contrast agent Levovist (containing microbubbles when reconstituted by adding saline and shaken) on the delivery of short DNA-FITC molecules into HeLa cells. The concentrations of cells and DNA-FITC were 2 x 10(5) cells/mL and 40 microg/mL, respectively. The cavitation was quantified as the inertial cavitation dose (ICD), corresponding to the spectral broadband signal enhancement during microbubble destruction. The relations of ICD with sonoporation and cell viability were examined for various acoustic pressures (0.48-1.32 MPa), Levovist concentrations (1.12 x 10(5)-1.12 x 10(7) bubbles/mL) and pulse durations (1-10 cycles). The linear regressions of the sonoporation rate versus ICD and the cell viability versus ICD were y = 28.67x + 10.71 (R(2) = 0.95) and z = -62.83x + 91.18 (R(2) = 0.84), respectively, where x is ICD, y is the sonoporation rate and z is the cell viability. These results show that the sonoporation rate and the cell viability are highly correlated with the ICD, indicating that sonoporation results may be potentially predicted using ICD.