教師著作
Permanent URI for this collectionhttp://rportal.lib.ntnu.edu.tw/handle/20.500.12235/37076
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Item Quantitative Relations of Acoustic Inertial Cavitation with Sonoporation and Cell Viability(Elsevier, 2006-12-01) C.-Y. Lai; C.-H. Wu; Chia-Chun Chen; P.-C. LiUltrasound-induced acoustic cavitation assists gene delivery, possibly by increasing the permeability of the cell membranes. How the cavitation dose is related to the sonoporation rate and the cell viability is still unknown and so this in vitro study quantitatively investigated the effects of cavitation induced by 1-MHz pulsed ultrasound waves and the contrast agent Levovist (containing microbubbles when reconstituted by adding saline and shaken) on the delivery of short DNA-FITC molecules into HeLa cells. The concentrations of cells and DNA-FITC were 2 x 10(5) cells/mL and 40 microg/mL, respectively. The cavitation was quantified as the inertial cavitation dose (ICD), corresponding to the spectral broadband signal enhancement during microbubble destruction. The relations of ICD with sonoporation and cell viability were examined for various acoustic pressures (0.48-1.32 MPa), Levovist concentrations (1.12 x 10(5)-1.12 x 10(7) bubbles/mL) and pulse durations (1-10 cycles). The linear regressions of the sonoporation rate versus ICD and the cell viability versus ICD were y = 28.67x + 10.71 (R(2) = 0.95) and z = -62.83x + 91.18 (R(2) = 0.84), respectively, where x is ICD, y is the sonoporation rate and z is the cell viability. These results show that the sonoporation rate and the cell viability are highly correlated with the ICD, indicating that sonoporation results may be potentially predicted using ICD.Item DNA-Gold Nanorod Conjugates for Remote Control of Localized Gene Expression by Near Infrared Irradiation(American Chemical Society, 2006-03-22) Chia-Chun Chen; Y.-P. Lin; C.-W. Wang; H.-C. Tzeng; C.-H. Wu; Y.-C. Chen; C.-P. Chen; L.-C. Chen; Y.-C. WuGold nanorods were attached to the gene of enhanced green fluorescence protein (EGFP) for the remote control of gene expression in living cells. The UV−vis spectroscopy, electrophoresis, and transmission electron microscopy (TEM) were used to study the optical and structural properties of the EGFP DNA and gold nanorod (EGFP−GNR) conjugates before and after femto-second near-infrared (NIR) laser irradiation. Upon NIR irradiation, the gold nanorods of EGFP−GNR conjugates underwent shape transformation that resulted in the release of EGFP DNA. When EGFP−GNR conjugates were delivered to cultured HeLa cells, induced GFP expression was specifically observed in cells that were locally exposed to NIR irradiation. Our results demonstrate the feasibility of using gold nanorods and NIR irradiation as means of remote control of gene expression in specific cells. This approach has potential applications in biological and medical studies.Item Preparation of Fluorescent Silica Nanotubes and their Application in Gene Delivery(Wiley-VCH Verlag, 2005-02-01) Chia-Chun Chen; Y.-C. Liu; C.-H. Wu; C.-C. Yeh; M.-T. Su; Y.-C. WuFluorescent silica nanotubes are synthesized through a sol–gel reaction using an anodic aluminum oxide membrane template. The nanotubes are filled with plasmid DNA encoding green fluorescence protein (GFP), which are incorporated into mammalian cells that subsequently express GFP (see Figure). The results demonstrate a novel application of nanotubes in biomolecule delivery.