利用小鼠模式研究慢性疼痛對於腸道菌群結構與其穩定性之影響

dc.contributor江殷儒zh_TW
dc.contributor陳建璋zh_TW
dc.contributorChiang, Yin-Ruen_US
dc.contributorChen, Chien-Changen_US
dc.contributor.author蒙國杰zh_TW
dc.contributor.authorBrandon Mong Guo Jieen_US
dc.date.accessioned2022-06-08T02:41:06Z
dc.date.available2022-01-28
dc.date.available2022-06-08T02:41:06Z
dc.date.issued2022
dc.description.abstractnonezh_TW
dc.description.abstractThe gut microbiota, a complicated and highly dynamic ecosystem, consists of microorganisms that live within the gastrointestinal tract of its hosts and its structure and stability is relevant to the host health. The gut-brain axis of vertebrates is a bi-directional integrated system composed by immune, endocrine, and neuronal components by which the knowledge regarding the interactions between the gut microbiota and the nervous system (e.g., brain) is lacking. For example, the influence of chronic muscle pain with or without peripheral nerve injury on gut microbiota has not been extensively addressed. Here we used spared-nerve injury (SNI) model that mimics neuropathic pain and acid-induced muscle pain (AIMP) model that mimics fibromyalgia to investigate the link between chronic muscle pain and the dynamics of gut microbiota in C57BL/6 mice. We cut the peripheral nerves for SNI model and performed repeated muscle insult on the left gastrocnemius muscle using acidic saline for AIMP model, evaluated with pain behavioral test, and collected multiple fecal samples to generate time-series data. High-throughput amplicon sequencing coupled with mothur identified certain bacteria genera such as Adlercreutzia, Bifidobacterium, Muribacullum, Oscillospira, Staphylococcus, and Erysipelatoclostridium significantly affected by the chronic pain treatments. Microbial interaction network analysis based on generalized Lotka–Volterra (gLV) equations with multiple simulations showed the instability of gut microbial community when the mice were exhibiting chronic pain. Moreover, the metagenome imputation shed light on the functional profiles of gut microbial communities. Altogether, our bioinformatic analyses of mice gut microbiota indicated the impact of chronic pain on the structure and stability of gut microbial community in vertebrates.en_US
dc.description.sponsorship生命科學系zh_TW
dc.identifier80650005S-40910
dc.identifier.urihttps://etds.lib.ntnu.edu.tw/thesis/detail/877c6074ce0a860eb1be22be4b557e82/
dc.identifier.urihttp://rportal.lib.ntnu.edu.tw/handle/20.500.12235/117155
dc.language英文
dc.subject腸道菌zh_TW
dc.subject疼痛zh_TW
dc.subject穩定性zh_TW
dc.subject交互作用zh_TW
dc.subject網絡zh_TW
dc.subjectmicrobiotaen_US
dc.subjectchronic painen_US
dc.subjectstabilityen_US
dc.subjectinteractionen_US
dc.subjectnetworken_US
dc.title利用小鼠模式研究慢性疼痛對於腸道菌群結構與其穩定性之影響zh_TW
dc.titleThe Impact of Chronic Pain on the Structure and Stability of Gut Microbial Community in Mouse Modelsen_US
dc.type學術論文

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