深層海水口服與外用對於二硝基氯苯誘導之異位性皮膚炎BALB/c小鼠模式的療效

Abstract

異位性皮膚炎是一種慢性的、發炎性皮膚疾病，其病症特徵有持續性的發炎反應和有搔癢感的皮膚損傷。本研究會在BALB/c小鼠的背部皮膚上持續地使用DNCB誘發類似異位性皮膚炎病症和傷害，並且評估使用深層海水對於由DNCB處理所誘發的類似異位性皮膚炎症狀及皮膚傷害是否具有改善的效果。深層海水富含營養物質和礦物鹽，並有著抗氧化和抗發炎的特性，或許可以做為發展成新的治療方式來為皮膚提供保護，抵抗異位性皮膚炎。因為深層海水獨特的特性，在現在的研究中，認為深層海水對於DNCB誘發之類似異位性皮膚炎症狀或許有治療的潛力。本研究的結果顯示在DNCB處理的口服深層海水組別，其表皮分數和肥大細胞數量有獲得改善的效果，而且在皮下血管白血球細胞浸潤以及脾臟形態的情形有緩和的趨勢。然而，在抓癢行為、表皮增厚、脾臟腫大以及IL-4含量方面並沒有顯著性的改善。DNCB處理的口服與外用深層海水萃取物的組別，其表皮分數、表皮增厚、肥大細胞數量和IL-4含量有獲得改善的效果，而且在皮下血管白血球細胞浸潤以及脾臟形態的情形有緩和的趨勢。然而，在抓癢行為、脾臟腫大方面並沒有顯著性的改善。結論，深層海水或許在改善異位性皮膚炎是一個有潛力的治療策略，其中又以同時口服深層海水以及外用深層海水萃取物抹劑的效果最好。

Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized with continuous inflammation and pruritic skin lesions. Repeated application of 2,4-dinitrochlorobenzene (DNCB) treated on the dorsal skin of BALB/c mice can induce AD-like symptoms and skin lesions. This study evaluated therapeutic effects of oral administration or oral combined topical application of deep ocean water (DOW) on DNCB-induced AD-like symptoms. DOW which is rich in micronutrients and minerals and with antioxidant and anti-inflammatory qualities, may be developed as an AD therapy to provide skin protection against AD. Because of the unique property of DOW, in recent studies, suggesting that its therapeutic potential on DNCB-induced AD-like symptoms in BALB/c mice. The present results showed that dermatitis score and number of mast cells was significantly alleviated in DNCB-treated oral administration groups, and infiltration of leukocytes and morphological changes in spleen were slightly attenuated. However, there was no significant difference in scratching behavior, epidermal thickness, splenomegaly and spleen IL-4 levels. In DNCB-treated oral administration and topical application groups, dermatitis score, epidermal thickness, number of mast cells and spleen IL-4 levels was significantly alleviated, and infiltration of leukocytes and morphological changes in spleen were slightly attenuated. However, there was no significant difference in scratching behavior and splenomegaly. In conclusion, these results suggest that DOW treatment may be a potentially therapeutic strategy in ameliorating atopic dermatitis. Furthermore, combination of oral administration and topical application of DOW had the best efficiency to ameliorate AD-like symptoms.