Quantitative analysis of multiple urinary biomarkers of carcinoid tumors through gold-nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry

dc.contributor 國立臺灣師範大學化學系 zh_tw
dc.contributor.author T.-R. Kuo en_US
dc.contributor.author J.-S. Chen en_US
dc.contributor.author Y.-C. Chiu en_US
dc.contributor.author C.-Y. Tsai en_US
dc.contributor.author C.-C. Hu en_US
dc.contributor.author Chia-Chun Chen en_US
dc.date.accessioned 2014-12-02T06:41:37Z
dc.date.available 2014-12-02T06:41:37Z
dc.date.issued 2011-08-05 zh_TW
dc.description.abstract A simple technique for quantitative analysis of four urinary biomarkers, tryptophan (TRP), 5-hydroxytryptophan (5-HTP), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) of carcinoid tumors is developed using gold nanoparticles as the assisted matrix in surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI–TOF MS). The optimal SALDI conditions for the efficient ionization of those biomarkers are systematically explored by the adjustments of the concentrations of gold nanoparticles and internal standards. The mass spectra with strong signals and minimal background noise are obtained using 1-naphthaleneacetamide (NAD) as the internal standard. The calibration curves of the biomarker concentrations are determined using SALDI–TOF MS and the high linearity is obtained in all samples. For future clinical testing, multiplexed detection of those biomarkers in the urine samples of healthy males is performed. The successful quantitative detections of TRP, 5-HTP, 5-HT and 5-HIAA indicate that our technique provided great potentials to be developed a simple and rapid platform for the tumor biomarker detections. en_US
dc.description.uri http://ac.els-cdn.com/S0003267011006519/1-s2.0-S0003267011006519-main.pdf?_tid=549d8452-b2f4-11e3-b3dc-00000aacb361&acdnat=1395625001_9a28eb65c23c2243f7418bf4578e23cb zh_TW
dc.identifier ntnulib_tp_C0301_01_078 zh_TW
dc.identifier.issn 0003-2670 zh_TW
dc.identifier.uri http://rportal.lib.ntnu.edu.tw/handle/20.500.12235/42362
dc.language en_US zh_TW
dc.publisher Elsevier Masson en_US
dc.relation Analytica Chimica Acta, 699(1), 81-86. en_US
dc.relation.uri http://dx.doi.org/10.1016/j.aca.2011.05.012 zh_TW
dc.subject.other Multiplexed assay en_US
dc.subject.other Gold nanoparticles en_US
dc.subject.other Surface-assisted laser desorption/ionization en_US
dc.subject.other Urinary biomarkers en_US
dc.subject.other 5-Hydroxyindole acetic acid en_US
dc.title Quantitative analysis of multiple urinary biomarkers of carcinoid tumors through gold-nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry en_US
Files
Collections