檢測UV-C LED抑制細菌及病毒之功效及建立檢測方法

Abstract

在日常生活中，四處含有致病菌株，當菌量少時可能對人沒特別影響，但菌量多的時候可能會引發疾病產生。近年來世界受到COVID-19的影響，因此消毒殺菌就顯得很重要。儘管目前疫情有減緩的趨勢，但並未完全消除COVID-19的威脅，加上擔心其他潛在的危險微生物，因此發展有效抑制細菌及病毒活性之日常消毒方法就顯得相當重要。本研究使用南亞光電提供之UV-C LED燈管進行其抑制致病菌株與病毒之確效實驗測試，檢測對象包括致病菌株 (大腸桿菌、產氣單胞菌、金黃色葡萄球菌ATCC 33591、綠膿桿菌ATCC 15442、克雷伯氏肺炎菌ATCC 10031)及腺相關病毒(adeno-associated virus，AAV)與慢病毒 (Lenti-virus)來進行活性抑制測試。測試結果顯示，在革蘭氏陰性菌方面，UV-C LED在照射距離110公分內約5-15分鐘可對大腸桿菌、親水性產氣單胞菌及綠膿桿菌可達到約90%以上的抑制效果。而克雷伯氏肺炎菌在照射距離110公分內1分鐘即可達到80%以上抑制效果。另外在革蘭氏陽性菌的金黃色葡萄球菌中，照射距離15公分射5分鐘可達到近60%抑制效果。在病毒方面，對沒有套膜的腺相關病毒在照射距離30公分內15分鐘可達80%抑制效果，對有套膜的慢病毒則在相同的照射距離下30分鐘達到約60%抑制效果。本研究建立一適用於一般實驗室之微生物抑制效果檢測方法，方便初步檢測未確效之新設備。In daily life, various environments harbor pathogenic strains of microorganisms. When the microbial load is low, it might not significantly affect humans, but a high microbial load can lead to the development of diseases. In recent years, the world has been impacted by COVID-19, highlighting the importance of disinfection and sterilization. While the pandemic has shown signs of slowing down, the threat of COVID-19 has not been completely eradicated. Coupled with concerns about other potential dangerous microorganisms, the development of effective daily disinfection methods that can inhibit bacterial and viral activity has become crucial. This study utilized UV-C LED lamps provided by Nan Ya Photonics Incoration to conduct efficacy tests on the inhibition of pathogenic bacteria and viruses. The tested subjects include pathogenic bacterial strains such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus (ATCC 33591), Pseudomonas aeruginosa (ATCC 15442), and Klebsiella pneumoniae (ATCC 10031), as well as adeno-associated virus (AAV) and lentivirus for activity inhibition tests. The test results demonstrated that concerning Gram-negative bacteria, UV-C LED achieves an inhibition effect of over 90% against Escherichia coli, Aeromonas hydrophila, and Pseudomonas aeruginosa within approximately 5-15 minutes of exposure at a distance of 110 cm. As for Klebsiella pneumoniae, an inhibition effect of over 80% is achieved within 1 minute of exposure at the same distance. Additionally, in the case of Gram-positive bacteria such as Staphylococcus aureus, a near 60% inhibition effect is attained with a 5-minute exposure at a distance of 15 cm. Regarding viruses, the naked adeno-associated viruses exhibited an 80% inhibition effect within 15 minutes of exposure at a distance of 30 cm.On the other hand, enveloped lentiviruses achieved an approximately 60% inhibition effect within 30 minutes of exposure at the same distance. This study establishes a microbiological inhibition effectiveness testing method suitable for general laboratories, facilitating preliminary examining of newly developed equipment that has not yet been validated.