以氧化鐵奈米粒子標記人類間質幹細胞誘導成為類神經細胞的追蹤表現及應用

dc.contributor吳忠信zh_TW
dc.contributorWu, Chung-Hsinen_US
dc.contributor.author盧珍妏zh_TW
dc.contributor.authorLu, Chen-Wenen_US
dc.date.accessioned2019-09-05T05:38:30Z
dc.date.available2017-07-17
dc.date.available2019-09-05T05:38:30Z
dc.date.issued2017
dc.description.abstract本研究的目的是以開發氧化鐵奈米粒子(ION)標記人類間質幹細胞 (MSCs)誘導體外分化成為類神經細胞 (NCs) 的應用以及在核磁共振成像(MRI)之追蹤表現。Ferucarbotran,一種臨床所使用的氧化鐵奈米粒子,此種陰性顯影劑可以在核磁共振成像下清楚的看見,因此被用來標記細胞內的追蹤觀察。本研究透過光學顯微鏡下發現體外培養的類神經細胞具有神經細胞的型態以及量測動作電位的功能表現。在光學顯微鏡下觀察到細胞呈現軸突樣的結構型態。這些類神經細胞比未分化的間質幹細胞表現較多頻率的動作電位。以氧化鐵奈米粒子標記對間質幹細胞的形態、功能和分化能力沒有影響。我們的結論發現,以體外誘導人類間質幹細胞 (MSCs)分化成的類神經細胞 (NCs) 表現較多頻率的動作電位,或許這些體外誘導生成的類神經細胞可以用於替代損傷的神經元。zh_TW
dc.description.abstractThe aim of the current study was to develop an iron oxide nanoparticle (ION) labelling and magnetic resonance imaging (MRI)-based protocol to allow visualization of the differentiation process of mesenchymal stem cells (MSCs) into neural-like cells (NCs) in vitro. Ferucarbotran, a clinically available ION, which can be visualized under MRI, is used for tracking cells implanted in vivo. The NCs were verified morphologically and histologically by light microscopy, and their functions were verified by measuring their action potentials. Conformational conversion of axon-like structures was observed under light microscopy. These NCs exhibited frequent, active action potentials compared with cells that did not undergo neural differentiation. The labelling of ION had no influence on the morphological and functional differentiation capacity of the MSCs. We conclude that the MSCs that were differentiated into NCs exhibited in vitro activity potential firing and may be used to replace damaged neurons.en_US
dc.description.sponsorship生命科學系zh_TW
dc.identifierG080043006S
dc.identifier.urihttp://etds.lib.ntnu.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=id=%22G080043006S%22.&%22.id.&
dc.identifier.urihttp://rportal.lib.ntnu.edu.tw:80/handle/20.500.12235/103959
dc.language英文
dc.subject氧化鐵奈米粒子zh_TW
dc.subject核磁共振成像zh_TW
dc.subject間質幹細胞zh_TW
dc.subject類神經細胞zh_TW
dc.subject動作電位zh_TW
dc.subjectiron oxide nanoparticle (ION)en_US
dc.subjectmagnetic resonance imaging (MRI)en_US
dc.subjectmesenchymal stem cells (MSCs)en_US
dc.subjectneural-like cells (NCs)en_US
dc.subjectaction potentialen_US
dc.title以氧化鐵奈米粒子標記人類間質幹細胞誘導成為類神經細胞的追蹤表現及應用zh_TW
dc.titleCharacterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cellsen_US

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