環境因子對稻米之泛素基因家族成員表現的影響
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Date
2007-06-??
Authors
張淑芝
孫智雯
Journal Title
Journal ISSN
Volume Title
Publisher
國立臺灣師範大學生命科學學系
Department of Life Science, NTNU
Department of Life Science, NTNU
Abstract
泛素是一種廣泛存在真核生物體內的高度保守性蛋白質，可參與調控各種生物功能，包括耗能性蛋白質降解作用、核酸修復、細胞週期循環，維持染色體結構、逆境反應、細胞間交互作用、各類細胞信息傳遞、內噬作用、病毒感染、致癌基因的調節、計畫性細胞凋亡、及植物生理反應的調節。而細胞內的泛素是從兩群基因家族成員所轉錄轉譯而成，這些成員包括聚合泛素基因及泛素延伸基因。為了瞭解稻米泛素基因家族成員的表現，是否會受到不同環境因素所影響，我們經由序列收集及比對分析，共找到了10個泛素基因家族成員，分別是3個聚合泛素基因及7個泛素延伸基因。再藉由反轉錄聚合酶連鎖反應的定量方法，來比較稻米的泛素基因群在不同環境因子下的表現差異。結果顯示熱處理促使osUBQ2和osUBQ9基因表現量增加；osUBQ3、osUBQ4以及osUBQ5基因傾向在幼苗組織中表現；而osUBQ7基因在營養器官中具有組織特定性表現。
Ubiquitin, a highly conserved protein in eukaryotic organisms, is generally participated in ATP-dependent proteolysis, DNA repair, cell cycle control, maintenance of chromatin structure, stress response, cell-cell interaction, signal transduction, endocytosis, viral infection, regulation of oncogenes, and regulation of plant physiological responses. Cellular ubiquitins are encoded by two types of ubiquitin gene families, polyubiquitin gene and ubiquitin extension gene. In order to understand whether the expression of rice (Oryza sativa L.) ubiquitin genes are regulated by different environmental factors, we first collect sequences of ubiquitin genes from several rice-related genome databases, and compare them in detail to avoid redundant gene sequences. We found 10 ubiquitin genes in rice genome, including 3 polyubiquitin genes and 7 ubiquitin extension genes. Furthermore, quantification of gene expression by RT-PCR technique indicated that these genes expressed differentially under different treatments. The heat-shock treatments increased the expression levels of osUBQ2 and osUBQ9 genes; the osUBQ3, osUBQ4, and osUBQ5 genes preferentially expressed in young seedlings; whereas osUBQ7 gene were specifically expressed in roots.
Ubiquitin, a highly conserved protein in eukaryotic organisms, is generally participated in ATP-dependent proteolysis, DNA repair, cell cycle control, maintenance of chromatin structure, stress response, cell-cell interaction, signal transduction, endocytosis, viral infection, regulation of oncogenes, and regulation of plant physiological responses. Cellular ubiquitins are encoded by two types of ubiquitin gene families, polyubiquitin gene and ubiquitin extension gene. In order to understand whether the expression of rice (Oryza sativa L.) ubiquitin genes are regulated by different environmental factors, we first collect sequences of ubiquitin genes from several rice-related genome databases, and compare them in detail to avoid redundant gene sequences. We found 10 ubiquitin genes in rice genome, including 3 polyubiquitin genes and 7 ubiquitin extension genes. Furthermore, quantification of gene expression by RT-PCR technique indicated that these genes expressed differentially under different treatments. The heat-shock treatments increased the expression levels of osUBQ2 and osUBQ9 genes; the osUBQ3, osUBQ4, and osUBQ5 genes preferentially expressed in young seedlings; whereas osUBQ7 gene were specifically expressed in roots.