Please use this identifier to cite or link to this item: http://rportal.lib.ntnu.edu.tw:80/handle/20.500.12235/104246
Title: 褐樹蛙(Buergeria robusta)的親緣地理學研究
Phylogeographic study of the brown tree frog (Buergeria robusta) in Taiwan
Authors: 林思民
Si-Min Lin
陳盈蓉
Ying-Rong Chen
Keywords: 族群遺傳
褐樹蛙
親緣地理學
Buergeria robusta
phylogeography
population genetics
Issue Date: 2010
Abstract: 親緣地理學(phylogeography)討論當物種受到歷史地質事件影響時,族群遺傳結構所產生的改變,並利用建構物種的親緣關係與調查族群在地理上的分佈,來檢視族群分化的歷史。在臺灣針對兩棲爬行動物的研究大多支持山脈為臺灣島上對物種分化而言最主要的地理屏障,然而目前卻尚未有針對褐樹蛙(Buergeria robusta)這種棲息生態同時受到陸域及淡水域影響的全島廣布物種進行親緣地理學的研究。本研究採集全島33個採樣點一共564隻褐樹蛙個體,以粒線體DNA中的細胞色素b(cytochrome b)基因片段做為分子遺傳標記,建構基因單型的親緣關係樹(phylogeny)與網狀譜系圖(network),討論褐樹蛙各族群間的遺傳分化程度及分布擴散歷程,並與前人研究的淡水魚類或其他小型兩棲爬行動物相比較。結果顯示褐樹蛙在親緣關係上可以分成七個支系,在地理分佈上可以分成五個區域。其中東部區域由於受到中央山脈的阻隔,與其他區域形成高度分化,而成為一個獨立的系群。在西部族群中,東北部、北部、中部與南部區域之間的遺傳交流極為有限,也各自擁有特殊的單基因型,而雪山山脈、苗栗丘陵與臺灣堆均成為阻礙基因交流的地理屏障。在族群歷史變動的部分,大部分主要遺傳支序皆呈現歷史上的分布範圍擴張,而大多數的地理區域則呈現歷史上的族群數量成長。總結而論,在臺灣造成物種產生遺傳分化的地理障礙,幾乎每一項都會成為褐樹蛙基因交流上的地理障礙,因此本研究認為褐樹蛙的遺傳分化模式兼具陸域生物與水域生物特徵而自成一格的特殊模式。
The Brown Treefrog, Buergeria robusta (Boulenger, 1909) is endemic to Taiwan and is widely-distributed throughout the island. In this study, phylogeographic pattern and population genetic structure of B. robusta were investigated based on 1080-bp mitochondrial cytochrome b sequences. A total of 564 samples of B. robusta specimens from 33 populations were analyzed. Phylogenetic tree and haplotype network reconstruction both showed that the entire species could be divided into the western and the eastern lineages, showing the role of the Central Mountain Range as a major barrier separating the western and eastern populations. The western populations could be further divided into 6 minor genetic clades and 4 subregions with limited gene flow. High-level nested clades showed strong tendency of historical range expansion and most regional populations showed evidences of population expansion in their geological history. Compared to other vertebrates in Taiwan, B. robusta shows high congruence with both terrestrial amphibians/reptiles and freshwater fishes, showing a combination of both terrestrial and water-living characteristics.
URI: http://etds.lib.ntnu.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=%22http://etds.lib.ntnu.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=id=%22GN0696430033%22.&%22.id.&
http://rportal.lib.ntnu.edu.tw:80/handle/20.500.12235/104246
Other Identifiers: GN0696430033
Appears in Collections:學位論文

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