念珠藻Nostoc punctiforme胞外多醣生合成調控機制之預測

dc.contributor.author曾志彧zh_tw
dc.contributor.author楊萌皓zh_tw
dc.contributor.author李冠群zh_tw
dc.contributor.authorChih-Yu Tseng, Ming-Ho Iunn, Guan-Chiun Leeen_US
dc.date.accessioned2022-05-16T07:47:25Z
dc.date.available2022-05-16T07:47:25Z
dc.date.issued2014-12-??
dc.description.abstract藍菌念珠藻屬念珠藻屬 (Nostoc) 的胞外多醣胞外多醣 (exopolysaccharides, EPS)生合成相關之遺傳與生化方面的瞭解,目前研究並不多,為探討,為探討念珠藻屬EPS生合成基因可能受到那些轉錄因子調控,進而了解EPS生合成與環境因子的關係,本研究以念珠藻屬中基因體序列已被完整解碼的Nostoc punctiforme ATCC 29133為研究對象,針對其基因體中10個可能參與EPS生合成的基因啟動子區域做序列分析,共預測出15種轉錄因子轉錄因子結合位,藉由與大腸桿菌(Escherichia coli) 轉錄因子結合位序列的相似性相似性比對,預測最有可能會調控EPS生合成基因的轉錄因子為ArgR、Fnr與LexA。最後,利用E. coli K12之ArgR、Fnr與LexA胺基酸序列於N. punctiforme基因體中進行BLAST比對搜尋,結果顯示N. punctiforme具有與具有與E. coliK12相似度極高的轉錄因子LexA,但不具有相似的ArgR與Fnr。因此推測念珠藻N.punctiforme的EPS生合成基因可能會受轉錄因子LexA與其相關環境因子的調控,例如紫外線。zh_tw
dc.description.abstractIn cyanobacteria Nostoc, the genetics and biochemistry of exopolysaccharide (EPS) biosynthesis have not been thoroughly investigated. To identify the transcription factors that may regulate EPS biosynthesis genes and to understand the relationship between the EPS biosynthesis and the environment factors, in this study, we carried out thein silico promoter analysis of ten EPS biosynthesis-related genes in Nostoc punctiforme ATCC 29133, whose genomic sequence has been determined. Total 15 putative transcription factor biding sites were found in the ten promoter regions. Three transcription factors, ArgR, Fnr, and LexA, were predicted to be the most possible ones by comparing binding site sequence similarity between E. coli K12 and N. punctiforme. Because no ArgR and Fnr homologs were found in N. punctiforme genome by the Basic Local Alignment Search Tool (BLAST) using the amino acid sequences of E. coli K12 ArgR, Fnr and LexA as query sequences, we predicted that N. Punctiforme EPS biosynthetic genes may be regulated by the transcription factor LexA and its associated environmental factors such as UV light.en_US
dc.identifier0BD32527-B38A-40B3-624D-B58E2AD1074C
dc.identifier.urihttp://rportal.lib.ntnu.edu.tw/handle/20.500.12235/115796
dc.language中文
dc.publisher國立臺灣師範大學生命科學學系zh_tw
dc.publisherDepartment of Life Science, NTNUen_US
dc.relation49,29-38
dc.relation.ispartof生物學報zh_tw
dc.subject.other藍菌zh_tw
dc.subject.other念珠藻Nostoc punctiformezh_tw
dc.subject.other胞外多醣胞外多醣zh_tw
dc.subject.other轉錄因子轉錄因子zh_tw
dc.subject.other啟動子zh_tw
dc.subject.otherCyanobacteriaen_US
dc.subject.otherNostoc punctiformeen_US
dc.subject.otherexopolysaccharideen_US
dc.subject.othertranscription factoren_US
dc.subject.otherpromoteren_US
dc.title念珠藻Nostoc punctiforme胞外多醣生合成調控機制之預測zh-tw
dc.title.alternativePutative gene regulation involved in the biosynthesis of Nostoc punctiforme exopolysaccharideszh_tw

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