低場核磁共振系統於乳癌組織檢測應用與特性研究

Abstract

本實驗參考過去實驗室，研究低場核磁共振系統，使用超導量子干涉元件（SQUID, superconducting uantum interference device）和預極化（prepolarization）技術的低磁場核磁共振系統（Low-field NMR）應用至腫瘤量測上。改進接收線圈，使SNR提高20 % (量測0.5 ml去離子水)，進而讓乳房樣品能有效進行微量（0.05 g ~ 0.2 g）量測，利用縱向鬆弛時間（T1）之物理方式量測，並進行乳房組織最小量測量與縮短實驗時間，乳房組織經由實驗後量測T1數值，統計資料證實，正常組織與腫瘤組織具有統計意義，並計算辨識正常組織與腫瘤組織的靈敏性(Sensitivity)與特異性(Specificity)，具有高度的數值，同時證明使用T1量測，進行腫瘤組織檢測可行性。最後整合病理報告，核磁共振與病理報告內的細胞活化標記(Ki-67)、病理分期具有相關性。藉此能快速又有效提供可量化數值，提供醫師一種生物組織檢測方式。In this study, a high-Tc SQUID-detected nuclear magnetic resonance spectrometer was set-up in National Taiwan University Hospital to discriminate Breast tumor. Improve Pick-up coil and let SNR increase 20 percent (measure 0.5 ml di-water). Make breast samples effective for micro-measurement (0.05 g ~ 0.2 g). Exploit physical measurements of longitudinal relaxation time（T1）to measure breast samples and then measurement of the minimum sample size of breast tissue and shortening the time of the experiment. Statistics confirmed that normal tissues and tumor tissues are statistically significant, and the sensitivity and specificity of identifying normal tissues and tumor tissues are calculated, with high numerical values, and it is proved that tumor tissue is measured using T1 measurement. For tumor discrimination, the T1 relaxation time of normal tissue and tumor tissue were demonstrated. Besides, the relation between T1 of tumor tissue and KI-67, pathology grade was established.