Mutational analysis of the hormone-sensitive lipase translocation reaction in adipocytes

dc.contributor 國立臺灣師範大學人類發展與家庭學系 zh_tw
dc.contributor.author Su, C.-L., Sztalryd, C., Contreras, J. A., Holm, C., Kimmel, A. R., and Londos, C., en_US
dc.date.accessioned 2014-12-02T06:40:04Z
dc.date.available 2014-12-02T06:40:04Z
dc.date.issued 2003-10-01 zh_TW
dc.description.abstract Lipolysis in adipocytes governs the release of fatty acids for the supply of energy to various tissues of the body. This reaction is mediated by hormone-sensitive lipase (HSL), a cytosolic enzyme, and perilipin, which coats the lipid droplet surface in adipocytes. Both HSL and perilipin are substrates for polyphosphorylation by protein kinase A (PKA), and phosphorylation of perilipin is required to induce HSL to translocate from the cytosol to the surface of the lipid droplet, a critical step in the lipolytic reaction (Sztalryd C., Xu, G., Dorward, H., Tansey, J. T., Contreras, J.A, Kimmel, A. R., and Londos, C. (2003) J. Cell Biol. 161, 1093–1103). In the present paper we demonstrate that phosphorylation at one of the two more recently discovered PKA sites within HSL, serines 659 and 660, is also required to effect the translocation reaction. Translocation does not occur when these serines residues are mutated simultaneously to alanines. Also, mutation of the catalytic Ser-423 eliminates HSL translocation, showing that the inactive enzyme does not migrate to the lipid droplet upon PKA activation. Thus, HSL translocation requires the phosphorylation of both HSL and perilipin. en_US
dc.description.uri http://www.jbc.org/content/278/44/43615.full.pdf zh_TW
dc.identifier ntnulib_tp_A0307_01_014 zh_TW
dc.identifier.issn 1083-351X zh_TW
dc.identifier.uri http://rportal.lib.ntnu.edu.tw/handle/20.500.12235/41478
dc.language en_US zh_TW
dc.publisher The American Society for Biochemistry and Molecular Biology, Inc. en_US
dc.relation Journal of Biological Chemistry, 278, 43615-43519. en_US
dc.relation.uri http://dx.doi.org/10.1074/jbc.M301809200 zh_TW
dc.title Mutational analysis of the hormone-sensitive lipase translocation reaction in adipocytes en_US
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