以光學同調斷層攝影術評估果蠅的心臟功能

Abstract

本研究提出用三維快速高解析度掃瞄式光學同調斷層儀(SS-OCT)系統以進行非侵入式活體成蟲果蠅心臟造影成像，分別量測正常標準種類之果蠅(Drosophila，w1118)，與心臟突變之果蠅(UAS-Acer、Mut8i、Mut9i、M8i、M9i、Δ164F1、Δ168M1)。並利用影像後處理的資訊取得果蠅各項心臟功能參數，如心跳率(Heart Rate，HR)、心臟舒張維度(end-diastolic dimension，EDD)、心臟收縮維度(end-systolic dimension，ESD)以及心肌短縮分率(fractional shortening，FS)，本研究目的包括(1)測試此套系統是否適用於清醒的成蟲果蠅之心臟功能精確量測；(2)判定果蠅是否為一個合適的重建人類心臟病的模式動物系統。In this study, a three dimensional fast scanning and high resolution Swept Source optical coherence tomography (SS-OCT) system for non-invasively imaging the heart in adult awake Drosophila of a standard strain (w1118) and mutation with cardiac function(UAS-Acer、Mut8i、Mut9i、M8i、M9i、Δ164F1、Δ168M1) is proposed. Heart functions such as Heart Rate (HR), end-diastolic dimension (EDD), end-systolic dimension (ESD), and fractional shortening (FS) were acquired by image post processing. The objectives of this study including: (1) to test whether the SS-OCT system could be applied to accurately measure cardiac function in awake adult Drosophila; (2) to determine whether the fly can be used as a model system to recapitulate human heart diseases.