Analysis of KLK1 Gene-130Gn Polymorphism with the Risk of Parkinson's Disease

dc.contributor.author黃淑宜zh_tw
dc.contributor.author莊蕙瑄zh_tw
dc.contributor.author陳瓊美zh_tw
dc.contributor.author吳逸如zh_tw
dc.contributor.author李桂楨zh_tw
dc.date.accessioned2014-10-27T15:02:13Z
dc.date.available2014-10-27T15:02:13Z
dc.date.issued2005-06-??zh_TW
dc.description.abstract帕金森氏症為一常見的神經退化性疾病,和遺傳及環境因素相關。KLK1為一serine protease,在人體包括大腦皮質、海馬迴等多處部位皆有表現。本研究的目的在探討KLK1基因啟動子-130G(下標 N)多型性與國人帕金森氏症感受性的相關性。-130G(下標 N)多型性基因型的分析方法包括螢光PCR產物的長度分析、單股核酸構形多型性/異雙股分析及DNA定序等。所研究樣品群包括208位帕金森氏症患者及95位正常人。結果共觀察到五種對偶基因:I (G9)、A (G10)、B (G2CG7)、H (G11)、K (Gl2)。多型性對偶基因的啟動子轉錄活性分析顯示,在人類胚胎腎細胞HEK-293及人類腦癌細胞IMR-32中,A、B、H對偶基因的轉錄活性並無顯著差異,但三者的轉錄活性均較K對偶基因顯著的好。進一步的依據含或不含K對偶基因的基因型,或K對偶基因頻率,進行X^2統計分析,結果顯示在正常人族群與帕金森氏症患者群間,並未呈現顯著差異。故推論KLK1基因啟動子多型性,雖然會影響其在人類胚胎腎細胞及腦癌細胞中的表現量,但其變異和國人帕金森氏症的感受性並沒有太大關連性。zh_tw
dc.description.abstractParkinson's disease (PD) is a common neurodegenerative disorder involving several genetic and environmental components. Human tissue kallikrein (KLK1) is a serine protease expressed in both the cerebral cortex and hippocampus. In this study v e investigated the association of KLK1 gene -130G(subscript N) polymorphism with the risk of Parkinson's disease. The -130G(subscript N) polymorphism as analyzed by electrophoresis of fluorescenced PCR products in sequencing gels. single-strand conformation polymorphism (SSCP)/heteroduplex analysis and DNA sequencing. Two hundred and eight patients with PD and 95 normal controls ere examined. Five alleles ere identified in the KLK1 promoter: I (G9), A (G10), B (G2CG7), H (G11), and K (G12). A reporter construct containing the K allele cloned into a luciferase reporter plasmid drove significantly lower transcriptional activity of KLK1 as compared with the A, B, and H alleles in both HEK-293 and IMR-32 cells. However, when the genotype and allele frequencies with or without K allele were analyzed, no statistically significant difference as observed between PD patients and controls. Therefore, the results indicate that although associated with lower transcriptional activity, KLK1 -130G(subscript N) K allele as not associated with the risk of Taiwanese PD.en_US
dc.identifierE7A62DA6-D435-1564-C9A5-219EAA83C754zh_TW
dc.identifier.urihttp://rportal.lib.ntnu.edu.tw/handle/20.500.12235/7130
dc.language中文zh_TW
dc.publisher國立臺灣師範大學生命科學學系zh_tw
dc.publisherDepartment of Life Science, NTNUen_US
dc.relation40(1),9-15zh_TW
dc.relation.ispartof生物學報zh_tw
dc.subject.other-130Gn多型性zh_tw
dc.subject.other帕金森氏症zh_tw
dc.subject.other疾病感受性zh_tw
dc.subject.otherKLK1en_US
dc.subject.other-130G[fed7] polymorphismen_US
dc.subject.otherParkinson's diseaseen_US
dc.subject.otherDisease susceptibilityen_US
dc.titleAnalysis of KLK1 Gene-130Gn Polymorphism with the Risk of Parkinson's Diseasezh-tw
dc.title.alternativeKLK1基因啟動子-130Gn多型性與帕金森氏症感受性的分析zh_tw

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