中斷久坐對健康者腹部與臀部皮下脂肪組織間隙血糖調控之影響

Abstract

研究背景：脂肪組織是人體中重要的內分泌器官、能量儲存庫，也在血糖的代謝上扮演著非常重要的角色。但目前的研究仍缺乏有無身體活動的介入下，比較不同部位中的脂肪組織對於血糖調控的差異性。研究目的：本研究目的主要探討健康者在有無中斷久坐腹部與臀部的皮下脂肪組織間質細胞血糖調控的影響。研究方法：20位健康參與者於皮下腹部與臀部脂肪組織分別安裝一台連續式血糖監測儀 (continuous glucose monitor, CGM)，並於安裝CGM七天內以平衡次序法完成時長約320分鐘的久坐與中斷久坐的實驗室測驗。久坐實驗中，參與者在整個實驗期間維持坐姿；中斷久坐實驗中，參與者以每20分鐘以6.4公里/小時的速度在跑步機上快走2分鐘，累積15次，總計30分鐘，其餘時間則維持坐姿。兩次實驗中皆提供兩餐相同的餐點 (早餐與午餐，每餐562 ± 81大卡 (平均數 ± 標準差)，含77 % 碳水化合物、16%脂肪、7%蛋白質)。研究結果：久坐實驗中，腹部皮下脂肪組織間隙血糖顯著高於臀部脂肪組織 (p = 0.03)。久坐與中斷久坐實驗中，腹部與臀部血糖的曲線下面積 (iAUC) 則無差異 (all, p> 0.05)。結論：本研究顯示健康者在久坐情境中，腹部的皮下脂肪組織間質細胞的血糖濃度顯著高於與臀部皮下脂肪組織，但在久坐與身體活動情境下，兩部位的血糖曲線下增加面積沒有差異。Background: Adipose tissue is not only responsible for storing excess energy as triglycerides, but also plays a vital role in regulating glucose homeostasis. However, it is still unknown regarding glycemic regulation in different adipose depots under (in)active status. Purposes: The study aims to investigate the differences between abdominal (Ab) and gluteal (Glu) adipose tissue interstitial glucose concentrations under sedentary (SIT) and physical activity (PA) conditions. Methods: Twenty healthy participants [age: 30 ± 8 yr; body mass: 63 ± 9 kg; BMI: 22.6 ± 2.1 kg/m2, (Mean ± SD)], with continuous glucose monitors (CGMs) inserted on Ab and Glu, completed two separate trials (SIT and PA) within 7 days under counterbalanced design. PA was composed of walking for 2 min at 6.4 km/h every 20 min over approximately 320 min (30 min walking in total), participants sat on a chair in the remained period apart from walking. In SIT, participants were asked to remain seated throughout the trial. Two identical meals (breakfast and lunch) [562 ± 81 kcal in one meal (Mean ± SD); 77% carbohydrate, 16% fat, 7% protein] were provided during trials. Results: Abdominal adipose tissue interstitial glucose concentrations were significantly higher than gluteal under SIT trial, while no regional difference was found under PA trial. There were no differences on incremental glucose area under the curve (iAUC) between Ab and Glu under SIT and PA trials in healthy individuals (all, p> 0.05). Conclusion: The current study showed subcutaneous abdominal adipose tissue interstitial glucose concentrations was significantly higher than gluteal region under SIT trial, while the glucose responses did not differ under acute sedentary compared to physical activity condition in healthy individuals.