間歇性增補OPC溶液對運動後氧化壓力的影響

Abstract

黃嘌呤氧化酶是造成運動中氧化壓力的主因，而寡聚原花青素 (oligo- meric proanthocyanidins, OPC)具有抑制黃嘌呤氧化酶的特性，因此本研究想了解間歇性增補OPC溶液對運動後氧化壓力的影響。本研究召募了16名健康國、高中足球選手（年齡15.95 ± 0.89歲；身高169.95 ± 5.6公分；體重57.75 ± 6.48 公斤），以PACER區分能力後，依平衡次序法分成實驗組與控制組。兩組受試者分別於跑前1小時增補一次，之後每隔2Km增補一次，同時於跑前15min (T1)、跑後5~10min內(T2)、跑後2小時(T3)及跑後24小時(T4)進行採血，以分析黃嘌呤氧化酶活性、尿酸生成量、肌酸磷化酶及丙二醛的變化情形。而運動強度的監控上則採用心率表及血乳酸值予以進行。所得資料以混合設計二因子變異數分析進行統計考驗並以Pearson積差相關考驗各變項間的相關性。結果發現：（1）增補OPC溶液可以減緩10Km跑過程所引起的黃嘌呤氧化酶及MDA的生成量，但無法降低尿酸與CPK的生成量。（2）黃嘌呤氧化酶的變化率與尿酸的生成量變化率達顯著正相關。由本實驗顯示雖然增補OPC溶液具有能減緩運動後黃嘌呤氧化酶活性與丙二醛濃度的趨勢，但不同劑量、不同增補方式都會影響其實驗結果。因此，未來有必要進一步再加以研究，以便能應用在運動保健之上。Oligomeric Proanthocyanidins (OPC) is a powerful anti-oxidative supplement, and it can depress the activity of xanthine oxidase (XOD), recently XOD was confirmed to be the major issue in increasing the oxidative pressure during exercise. The purpose of this study was to examine the effects of OPC solution supplementation on the exercise induced oxidative stress. Sixteen junior and senior male soccer players (age=15.95 ± 0.89 years; Height=169.95 ± 5.6 cm; Weight=57.75 ± 6.48Kg), To discriminate ability by PACER, grouping by counter-balanced order.Using 10 Km run to induce oxidative stress and monitor intensity by polar and blood lactic acid. Expriment group supplements OPC solution (120 ml H2O + 5mg/kg OPC powder), control group supplements placebo (120 ml H2O + concentrate grape juce 1 ml). Supplement time were set at 1hour before running and each 2Km during running. Blood samples were collected at 15 min before running (T1), 5~10min after running (T2), 2 hours after running (T3), and 24 hours after running (T4)。The change ratio of XOD and the concentration of uric acid, CPK, and MDA were measured. All data were analyzed using mixed design two-way ANOVA and Pearson product-moment correlation. The results showed that : 1) OPC solution supplementation could diminish the production of XOD and MDA induced by running, but not uric acid and CPK. 2) The change ratio of uric acid concentration and xanthine oxidase concentration was singnifcant positive correlation (p＜ .05). We concluded that the dosage and method of OPC solution supplementation might influence the variation on the oxidative stress induced by strenuous training. It’s necessary to research in depth for health care.