Opto-mechanical analysis for confocal laser scanning microscopes

dc.contributor 國立臺灣師範大學機電工程學系 zh_tw
dc.contributor.author Chang, Gao-Wei en_US
dc.contributor.author Twu, Ming-Jenq en_US
dc.contributor.author Lin, Yu-Hsuan en_US
dc.contributor.author Liao, Chia-Cheng en_US
dc.contributor.author Kuo, Hung-Zen en_US
dc.date.accessioned 2014-10-30T09:36:25Z
dc.date.available 2014-10-30T09:36:25Z
dc.date.issued 2005-08-02 zh_TW
dc.description.abstract The confocal imaging has become one of the most widely applied microscopic techniques in various fields, such as biotechnology, automation engineering, optical engineering, solid-state physics, metallurgy, integrated circuit inspection, etc. Confocal laser scanning microscopy (CLSM) is primarily based on the use of apertures in the detection path to provide the acquired three-dimensional images with satisfactory contrast and resolution. The major objective of this paper is to analyze the imaging performance of the confocal microscopes with varying opto-mechanical conditions. In this paper, the working principles of the one- and two-dimensional scanning mechanisms in the microscopic systems are first reviewed and verified by opto-mechanical simulations. Then, for the imaging performance, the tolerance to the fabrication and assembly of the optical components in conventional confocal microscopes is also investigated by simulations. The simulation results indicate the importance of eliminating the effects of stray light in the microscopic systems. en_US
dc.identifier ntnulib_tp_E0405_02_001 zh_TW
dc.identifier.uri http://rportal.lib.ntnu.edu.tw/handle/20.500.12235/37061
dc.language en zh_TW
dc.publisher The International Society for Optical Engineering (SPIE) en_US
dc.relation Proceedings of the SPIE 5877, 587710, Optomechanics, San Diego, CA. en_US
dc.relation.uri http://dx.doi.org/10.1117/12.616640 zh_TW
dc.title Opto-mechanical analysis for confocal laser scanning microscopes en_US