主動式色變電漿子可視化生醫感測晶片之研發

Abstract

傳統的SPR檢測儀器擁有即時檢測、免標的、靈敏度高等優點，本研究將傳統的SPR檢測儀器積體微型化設計，提出新型主動式色變電漿子可視化生醫晶片，用於定點照護檢測、即時檢測與早期診斷提高現今社會的醫療品質。奈米壓印顯影技術是一種簡易製程技術，此技術應用於奈米結構的複印具有成本低，產量高等優勢，對於日後主動式色變電漿子生醫晶片的製造有極高的產業化優勢。主動層以有機發光二極體具備放光強度的均勻性，在電漿子生物檢測晶片的檢測過程中是不可或缺的條件。 利用奈米壓印製作的奈米光柵激發表面電漿波，以下分別將實驗分為三大類，實驗一：奈米光柵於不同折射值環境內的共振角位移及色變能力，實驗二：金奈米粒子增強表面電漿量測生物分子訊號的靈敏度，實驗三：結合有機放光二極體的主動式色變電漿子晶片於生物反應檢測。 光柵週期555 nm的結構，當折射值從n = 1到1.33，其耦合波長位移約190 nm，得到最低折射值變化量為1.736×10-3 RIU。利用金奈米粒子(15 nm)鍵結Peptide (1 mM)後的反應液與hCG (1 µM)鍵結反應，相較於未添加金奈米粒子的反應提高約43.88倍，其最低折射值變化量的靈敏度提高至3.956×10-5 RIU。相同條件下，使用金奈米粒子(33 nm)鍵結Peptide後與hCG特異性鍵結的參數用於主動式色變電漿子晶片中，固定量測角度：5°，因為環境折射值改變，以致電漿子放光波長位移16 nm，固定量測角度：-5°，其電漿子放光波長位移4 nm。我們預期色變電漿子生醫晶片未來可用於疾病的診斷，國人的健康是國家成長的動力，診斷科技可以改善人類於早期診斷及提早治療。

The traditional SPR instrument has the advantages of real time, label free and high sensitivity. We proposed a new active color-plasmonic naked-eye biosensors that made the traditional SPR instrument miniaturization for point of care testing (POCT) with immediate detection and early diagnosis to improve the medical quality of today's society. Nano-imprinting technology is a simple process technology, this technology used in the preparation of nano-structure with low cost, high yield advantages that active color-plasmoinc biosensors manufacturing has a high production advantage in the future. Organic light-emitting diodes have uniformity of light emission intensity, which is an indispensable condition in the detection process of active color-plasmonic naked-eye biosensors. In this article that using nano-grating to excite the surface plasmonic wave.The experiment was divided into three categories. Experiment 1: The SP angular displacement and the colorimetric ability of the nano-grating in different refractive index environments. The sensitivity of the biomolecule signal of the nanoparticle to enhance the measurement of the biomolecule signal. Experiment 3: Testing active color-plasmonic biosensors combined in the biomolecule reaction. When the refraction index is from n = 1 to 1.33, the coupling wavelength shifted about 190 nm and the minimum refractive index is 1.736 × 10-3 RIU. The reaction of the reaction solution with gold nanoparticles (15 nm) after peptide (1 mM) was combined with hCG (1 μM), which was about 43.88 times higher than that of the metal nanoparticles without adding gold nanoparticles. The sensitivity was increased to 3.956 x 10-5 RIU. Under the same conditions, the use of gold nanoparticles (33 nm) bonding Peptide and hCG specific binding parameters for active color-plasmonic biosensors.Measurement angle: 5 °, because the ambient refraction index changes So that the emission shifted of plasmonic emission light 16 nm.Measurement angle: -5 °, the plasmonic emission wavelength shift 4 nm. We expect the active color-plasmon biosensors to be used in the future for the diagnosis of disease.The health of the people is the driving force of national growth and diagnostic technology can improve the early diagnosis and early treatment of human.