二枚貝之血淋巴球分類與形態觀察
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Date
2011-06-??
Authors
洪紹文, 張永昌,陳明輝,鄭清福,涂青宇,林育興,林荀龍,劉邦成,張鎮璿,鄒禮澤,王渭賢
Shao-Wen Hun1, Yung-Chung Chang, Ming-Hui Chen, Chin-Fu Cheng, Ching-Yu Tu,Yu-Hsing Lin, Shiun-Long Lin, Pan-Chen Liu, Chen-Hsuan Chang, Li-TseTsou,Way-Shyan Wang
Journal Title
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Volume Title
Publisher
國立臺灣師範大學生命科學學系
Department of Life Science, NTNU
Department of Life Science, NTNU
Abstract
文蛤 (hard clam, Meretrix lusonia Roding) 和台灣蜆 (freshwater clam, Corbicula flumineaMullar) 皆為本省重要的養殖貝類，養殖過程中常因養殖環境惡化，使得產量極不穩定，造成嚴重的經濟損失。許多因素皆可造成貝類死亡與生長遲緩，其中微生物感染亦包括其中。因此，如何降低死亡與增加產量為當務之急。本實驗目的在於研究兩種二枚貝類 (文蛤與台灣蜆) 血淋巴球之分類與形態觀察。利用percoll 梯度離心並輔以光學與電子顯微鏡觀察，結果顯示文蛤之血淋巴球可分為3 種血球：嗜鹼性顆粒球 (density: 1.055 g/mL)、嗜酸性顆粒球 (density: 1.029 g/mL) 及透明血球 (density: 1.018 g/mL)。於台灣蜆之血淋巴球亦有類似之嗜鹼性顆粒球 (density: 1.067 g/mL)、嗜酸性顆粒球 (density: 1.047 g/mL) 及透明血球 (density: 1.020 g/mL)。這些結果對於貝類血淋巴球之研究，可以此為基礎，並進一步地研究貝類之各類血淋巴球之細胞性免疫的功能與作用，以及進一步地瞭解其非特異性免疫的特性。因此，基於以上對貝類血淋巴球的初步研究，希望對於未來貝類疾病預防及防治上能有所助益。
Both of hard clam (Meretrix lusonia Roding) and freshwater clam (Corbicula fluminea Mullar) areimportant cultural shellfishes in Taiwan. During cultural period, environmental deterioration ofteninduced the unstable production of shellfish and caused serious economic losses. Therefore, how toreduce the death of clams and increase production is a priority. Many factors like as microbial infectioncan cause deaths and growth delay of shellfish. Therefore, how to decrease the mortality and increasethe production of shellfish is a priority. Aim of this study is to study the classification and morphologyof shellfish (hard clam and fresh clam) haemocytes. By percoll gradient centrifugation andsupplemented by optical and electron microscopy experiments showed that haemocytes of hard clamscan be divided into three cellular kinds: basophilic granulocyte (density: 1.055 g/mL), eosinophilicgranulocyte (density: 1.029 g/mL) and transparent cells (density: 1.018 g/mL). Fresh clams alsoshowed the similar results: the basophilic granulocyte (density: 1.067 g/mL), eosinophilic granulocyte(density: 1.047 g/mL) and transparent cells (density: 1.020 g/mL). These results for the future studiesin shellfish haemocytes can be used as the basis then perform the further study of cellular immunityand non-specific immunity of these cells. Therefore, we hope that disease prevention and control of theshellfish can be helpful in the future based on the above preliminary investigation of shellfishhaemocytes.
Both of hard clam (Meretrix lusonia Roding) and freshwater clam (Corbicula fluminea Mullar) areimportant cultural shellfishes in Taiwan. During cultural period, environmental deterioration ofteninduced the unstable production of shellfish and caused serious economic losses. Therefore, how toreduce the death of clams and increase production is a priority. Many factors like as microbial infectioncan cause deaths and growth delay of shellfish. Therefore, how to decrease the mortality and increasethe production of shellfish is a priority. Aim of this study is to study the classification and morphologyof shellfish (hard clam and fresh clam) haemocytes. By percoll gradient centrifugation andsupplemented by optical and electron microscopy experiments showed that haemocytes of hard clamscan be divided into three cellular kinds: basophilic granulocyte (density: 1.055 g/mL), eosinophilicgranulocyte (density: 1.029 g/mL) and transparent cells (density: 1.018 g/mL). Fresh clams alsoshowed the similar results: the basophilic granulocyte (density: 1.067 g/mL), eosinophilic granulocyte(density: 1.047 g/mL) and transparent cells (density: 1.020 g/mL). These results for the future studiesin shellfish haemocytes can be used as the basis then perform the further study of cellular immunityand non-specific immunity of these cells. Therefore, we hope that disease prevention and control of theshellfish can be helpful in the future based on the above preliminary investigation of shellfishhaemocytes.