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科技與工程學院
光電工程研究所
學位論文
學位論文
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http://rportal.lib.ntnu.edu.tw/handle/20.500.12235/73896
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search.filters.author.葉仲禹
1
search.filters.author.Yeh, Chung- Yu
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search.filters.subject.Digital Holography
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search.filters.subject.Living Cells Measurement
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search.filters.subject.Micro-Incubator
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search.filters.subject.數位全像術
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search.filters.subject.活細胞量測
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生物微培養器研製及其應用於數位全像顯微活細胞觀測
(
2022
)
葉仲禹
;
Yeh, Chung- Yu
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本研究為研製生物微培養器系統維持生物細胞存活,並運用數位全像顯微鏡(Digital Holography Microscope, DHM)進行長時間(72 hrs.)活細胞量測,為了達到長時間連續觀測活細胞,本論文研製出可以放在顯微系統上的微型生物培養器系統。研製之微型培養器解決了現階段常會遇到實務應用上的問題,例如:溫控加熱不均、體型過大,沒有氣體系統導致能培養的細胞種類有所限制、成本過高等等,此研究運用微型控制器、低電壓電路設計與3D列印的技術,可依照量測系統做客製化設計,且改善製作成本、具有安全性,也能提供多種細胞生長、分裂之環境的生物微培養器,搭配數位全像顯微系統進行長時間的細胞觀測與造影,本研究實驗結果可驗證此系統具實務可行性,並可應用於觀測細胞分裂等結果。
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