光學影像技術於生物組織之研究

Abstract

光學影像技術具有價格低廉、快速顯像、可攜性等優點。本研究可分為兩部分，首先，我們建立一套頻域式光學同調斷層攝影系統，用以取得生物組織的背向散射訊號。其利用同調光源於異質性之組織，藉由獲得組織其不同折射率之背向散射訊號，致使重建組織之結構影像。 最後，我們有效利用都普勒光學微血管攝影術於仿體以及老鼠表皮血管分佈之成像。此外，並利用此技術於老鼠大腦之血管攝影實驗，使有效地成像出鼠腦之血管分佈影像。Optical imaging technique has the advantages of cost-effectiveness, non-invasive, rapid imaging and portability. In this thesis, we have two topics. Firstly, we developed a spectral-domain optical coherence tomography system to obtain biological tissue scattering, the system uses coherence gating of backscattered light for tomographic imaing of tissue structure. Variation in tissue scattering due to in-homogeneities in the optical index of refraction provide imaing contrast. Finally, we have successfully used Doppler optical micro-angiography (DOMAG) to image phantom flow and skin blood perfusion in mice. We then conduct in vivo experiments on a mouse brain to demonstrate that DOMAG is capable of quantifying the blood flow within cerebrovascular network.