極化光學同調斷層攝影術(PS-OCT)對人體血管組織光學特性之定量分析

Abstract

在本研究中，我們開發了一套演算法，可從PS-OCT所量測到的二維影像中取得四個不同的光學參數，包括散射係數、非均向性係數、雙折射係數，以及快軸夾角。 散射係數與非均向性係數可用來描述樣品的散射效應和非均向性；雙折射特性指的是組織中快、慢軸折射率的差異；快軸夾角可以描述組織的纖維結構排列方向。首先，我們使用了一些仿體對演算法進行過測試與校正後，接著我們證明了使用這個方法可以量化的描述人體血管在正常與不同病變類型情況下的光學特性。此外我們也詳細討論了在不同組織中的雙折射特性。 In this study, we developed an algorithm to extract four different optical parameters including scattering coefficient, anisotropy factor, birefringence coefficient, and fast axis angle from polarization-sensitive optical coherence tomography (PS-OCT) images. Scattering coefficient and anisotropy factor can be used to describe scattering effect and anisotropy characteristic of the sample. Birefringence property represents the difference in tissue refractive index between the birefringent fast and slow axes, while fast axis angle can describe the fibrous arrangement of the fibrous structure in the tissue. First, we use phantom to test and correct our algorithm in this study. And then, we demonstrate that the optical characteristic of normal and different kinds of lesions in human vessel can be quantitative described by this method. In addition, we also discuss the birefringence properties of several different tissues in detail.