國立臺灣師範大學化學系Y.-Y. HorngY.-K. HsuA. GangulyChia-Chun ChenL.-C. ChenK.-H. Chen2014-12-022014-12-022009-04-011388-2481http://rportal.lib.ntnu.edu.tw/handle/20.500.12235/42340Amperometric enzyme biosensor based on the glucose oxidase (GOx) incorporated polyaniline nanowires (PANI-NWs) on carbon cloth (CC) electrode was demonstrated. The simple, direct-growth of PANI-NWs on CC, via electrochemical polymerization, provides free-standing, template-independent, hence almost (interfacial) defects-free nanostructures. The defect-free interfaces, along with the excellently sensitive organic nanostructured-surface, as evident from its significantly large effective surface area (24 times the geometric area) for redox-sensing, allows efficient entrapment/immobilization and sensing of biomolecules, via rapid electron-transfer at NWs-CC. The GOx-immobilized PANI-NWs/CC, even in initial unoptimized stage, exhibited an excellent sensitivity, ∼2.5 mA mM−1 cm−2, to glucose, over detection range 0–8 mM, adequate for clinical monitoring of human glucose levels. The report clearly reveals a cost-effective simple system possessing enormous potentiality for biosensors, bioenergy and bioelectronics applications.Polyaniline (PANI) nanowiresElectrochemical polymerizationNerstian behaviorGlucose oxidaseGlucose biosensor