葉怡均Yeh, Yi-Chun張嘉雯Chang, Chia-Wen2023-12-082023-07-312023-12-082023https://etds.lib.ntnu.edu.tw/thesis/detail/de9ed2b25f41fd5999e90788f0f5f0ce/http://rportal.lib.ntnu.edu.tw/handle/20.500.12235/121020我們實驗室提出以全細胞生物感測器來有效檢測並定量水中的硝酸鹽及亞硝酸鹽的方法，參考葡萄糖在大腸桿菌中的代謝路徑建構之質體轉殖入 E. coli BL21(DE3)，其製造的T7 RNA polymerse (RNAP)會開啟T7啟動子製造下游蛋白，這些蛋白會加速葡萄糖代謝成為4-Aminobenzoic acid (PABA)，使我們在短時間內就可以得到大量PABA，其經由一系列反應得到最終產物在540 nm有最大吸收的色素，以吸收回推濃度、再以大腸桿菌還原硝酸鹽為亞硝酸鹽後並以同樣反應定量出硝酸鹽濃度。In this study, we propose a method for the efficient detection and quantification of nitrate and nitrite in water using biosynthesis product 4-Aminobenzoic acid (PABA). Referring to the metabolic pathway of glucose in E. coli, the plasmid constructed is transformed into E.coli BL21(DE3). The T7 RNA polymerse (RNAP) produced by E. coli BL21(DE3) will turn on the T7 promoter to produce downstream proteins, which will accelerate glucose metabolism into PABA, so that we can obtain a large amount of PABA in a short time. PABA passes through a series of reactions to obtained the pigment as final product, which the maximum absorbance is at 540 nm. The concentration is calculated by the absorbance, and the nitrate is reduced by E. coli and quantified by the same reaction.硝酸鹽亞硝酸鹽定量檢測比色法nitratenitritequantitative detectioncolorimetryetd