國立臺灣師範大學化學系C.-C. LinY.-C. YehC.-Y. YangC.-L. ChenG.-F. ChenChia-Chun ChenY.-C. Wu2014-12-022014-12-022002-04-100002-7863http://rportal.lib.ntnu.edu.tw/handle/20.500.12235/42294The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.