林震煌Cheng-Huang Lin陳冠甫Kuan-Fu Chen2019-09-042018-6-192019-09-042013http://etds.lib.ntnu.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=id=%22GN0699420326%22.&%22.id.&http://rportal.lib.ntnu.edu.tw:80/handle/20.500.12235/100932本研究利用微波輔助螢光標定應用於毛細管電泳分析方法，用來分析鄰、間、對-氯安非他命和鄰、間、對-氟安非他命這六種安非他命濫用藥物與 2,5-dimethoxy- 4-ethylthio-phenethylamine（2C-T-2）、2,5-dimethoxy-4-（n）-propylthiophen- ethylamine（2C-T-7）、4-chloro-2,5- dimethoxyphenethylamine（2C-C）、4-bromo-2,5-dimethoxy-phenethylamine（2C-B）及 2,5-dimethoxy-4-iodo- phenethylamine（2C-I）五種 2C 系列的苯乙胺類毒品。利用 FITC（fluorescein isothiocyanate isomer I）當作螢光標記並使用藍光雷射（473 nm）作激發光源進行偵測。在過去使用 FITC 作衍生劑的衍生時間至少需要 20 小時，現今使用微波輔助衍生所需時間約 5 分鐘即可完成。A microwave-assisted fluorescence labeling method for use in CE-LIF （capillary electrophoresis-laser induced fluorescence） is described. Six amphetamine-like designer drugs, namely, o-, m-, p-chloro- and o-, m-, p-fluoro-amphetamine derivatives, and five 2C-series of phenethylamine designer drugs, including 2,5-dimethoxy-4-ethylthio-phenethylamine （2C-T-2）, 2,5-dimethoxy-4-（n）-propylthiophenethylamine（2C-T-7）, 4-chloro-2,5-dimethoxy-phenethylamine（2C-C）, 4-bromo-2,5-dimethoxy- phenethylamine（2C-B）, 2,5-dimethoxy-4-iodo-phenethylamine（2C-I） derivatives, were synthesized and used as model compounds. FITC （fluoresceinisothiocyanate isomer I） and a blue-laser were used as the fluorescent labeling reagent and excitation source, respectively. When a microwave oven was used, the reaction was complete within ~ 5 min,while the classical method required at least 20 h（usually, an overnight reaction）毛細管電泳微波衍生安非他命濫用藥物苯乙胺類FITCCE-LIFmicrowave-assistedamphetamine-like designer drugsphenethylamine designer drugs