陳家俊Chen, Chia-Chun鐘冠蘭Chung, Kuan-Lan2019-09-04不公開2019-09-042017http://etds.lib.ntnu.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=id=%22G060442100S%22.&%22.id.&http://rportal.lib.ntnu.edu.tw:80/handle/20.500.12235/100163螢光金奈米團簇（Gold Nanoclusters, Au NCs）具有獨特的光學性質、易於進行表面修飾及良好的生物相容性等優點，使其具有生物顯影及生物感測之應用性。本研究利用穀胱甘肽做為還原劑製備出發橘紅色螢光之金奈米團簇，並以β-D硫代葡萄糖（1-Thio-β-D-glucose）修飾其表面，合成出表面修飾葡萄糖之螢光金奈米團簇（TGlu-Au NCs）。我們利用顯微注射及餵食的方式，使TGlu-Au NCs進入秀麗隱桿線蟲（C. elegans）體內，在線蟲的咽、體腔及肛門都能清楚看到TGlu-Au NCs的螢光成像。使用大腸桿菌（E. coli）觀察TGlu-Au NCs的螢光變化，得到大腸桿菌濃度上升會使TGlu-Au NCs螢光強度下降之結果，顯示TGlu-Au NCs做為生物感測器之潛力。另將腦瘤細胞之糖酵解酶取出與TGlu-Au NCs反應，發現TGlu-Au NCs的螢光強度隨著反應時間愈長而下降愈多，顯示腫瘤細胞發生糖解作用，證實TGlu-Au NCs可利用腦瘤細胞的瓦氏效應行標記之作用。Fluorescent gold nanoclusters have been widely employed in the bioimaging and biosensing because of their unique optical properties, facile surface modification and high biocompatibility. In this study, we used L-Glutathione as a reductant to prepared fluorescent gold nanoclusters (Au NCs) and then the surface of Au NCs was modified with 1-Thio-β-D-glucose (TGlu-Au NCs). TGlu-Au NCs were respectively microinjected and fed into C. elegans to investigate their in vivo fluorescence imaging. The fluorescence of TGlu-Au NCs was easily visible in the lumen of the digestive system, extending from the pharynx to the anal region. Furthermore, the fluorescence changes of TGlu-Au NCs were studied with E. coli. TGlu-Au NCs at 0.4 (O.D.) showed higher decreasing rate of fluorescence intensity than that of TGlu-Au NCs at 0.27 (O.D.). In addition, the glycolytic enzyme of brain tumor cells (U-87 MG) was added into TGlu-Au NCs solution. After the reaction with the glycolytic enzyme, the fluorescence intensity of TGlu-Au NCs was decreased with the increase of time. The results confirmed the Warburg effect of TGlu-Au NCs in brain tumor cell.螢光金奈米團簇表面修飾1-Thio-β-D-glucose螢光成像生物感測瓦氏效應Fluorescent Gold NanoclustersSurface Modification1-Thio-β-D-glucoseFluorescence ImagingBiosensorWarburg Effect