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Department of Life Science, NTNU
Shy-Jiun-Tsi-Tang is mainly administerred by oral. Therefore, the mucosal immune system on the gastrointestinal tract should be the major area that is directly stimulated by the drug. Since the Immunoglobulin △ (IgA) secretion was considerred as a indicator of the mucosal immune function, the experimental design in this study is to investigate the possible regulatory effect of Shy-Jiun-Tsi-Tang and its major ingradients on the IgA secretion by human B lymphocytes. Results indicated that Shy-Jiun-Tsi-Tang might enhanced IgA secretion by unfractionated human peripheral B lymphocytes. However, the same treatment resulted in a drastic cytotoxicity to the unfractionated human tonsil B lymphocytes togather with a significant decrease in the amount of 19A secretion. When the unfractionated B lymphocytes were replaced by the highly purified 13 lymphocytes, the drug treatment also resulted in cytotoxicity and a significant reduction in 19A secretion. An IgA secreting human Iymphoblastoid cc1lline, Dakiki, was subsequently assayed for its sensitivity to the dmg. Results suggested that the drug caused a significant cytotoxicity but an increase in IgA secretion. The study therefore concluded that Shy-.liun-Tsi-Tang showed cytotoxic effect to the 13 lymphocytes in the absense of T lymphocytes and monocytes However, with the help of T lymphocytes and monocytes, the dmg might augment 19A secretion by B lymphocytes without showing cytotoxic effect. In addition, the dmg showed cytotoxic effect on the transformed B Iymphoblasts but enhanced IgA secretion by the same cell lines.
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