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Title: Globotriose-Functionalized Gold Nanoparticles as Multivalent Probes for Shiga-like Toxin
Authors: 國立臺灣師範大學化學系
Y.-Y. Chien
M.-D. Jan
A.-K. Adak
H.-C. Tzeng
Y.-P. Lin
Y.-J. Chen
K.-T. Wang
C.-T. Chen
Chia-Chun Chen
C.-C. Lin
Issue Date: 5-May-2008
Publisher: Wiley-VCH Verlag
Abstract: Compared to monovalent carbohydrates, multivalent carbohydrate ligands exhibit significantly enhanced binding affinities to their interacting proteins. Here, we report globotriose (Pk ligand)-functionalized gold nanoparticle (AuNP) probes for the investigation of multivalent interactions with the B5 subunit of Shiga-like toxin I (B-Slt). Six Pk-ligand-encapsulated AuNPs (Pk-AuNPs) of varying particle size and linker length were synthesized and evaluated for their potential as multivalent affinity probes by using a surface plasmon resonance competition assay. The affinity of these probes for the interacting proteins was greatly affected by nanoparticle size, linker length, and ligand density on nanoparticle surface. For example, the 20-nm 20-Pk-l-AuNP, which had a relatively long linker showed a >108-fold increase in affinity compared with the mono Pk ligand. This intrinsic high-affinity AuNP probe specifically captured the recombinant B-Slt from Escherichia coli lysate, and the resulting purity of the B-Slt was >95 %. We also developed a robust Pk-AuNP-based detection method for Slt-I by combining the technique with silver enhancement.
ISSN: 1439-4227
Other Identifiers: ntnulib_tp_C0301_01_052
Appears in Collections:教師著作

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