Please use this identifier to cite or link to this item: http://rportal.lib.ntnu.edu.tw:80/handle/77345300/41470
Title: Morusin induces apoptosis and suppresses NF-kappaB activity in human colorectal cancer cells
Authors: 國立臺灣師範大學人類發展與家庭學系
Lee, J.-C., Won, S.-J., Chao, C.-L., Wu, F.-L., Liu, H.-S., Ling, P., Lin, C.-N., and Su, C.-L.,
Issue Date: 1-Jul-2008
Publisher: Elsevier
Abstract: Morusin is a pure compound isolated from root bark of Morusaustralis (Moraceae). In this study, we demonstrated that morusin significantly inhibited the growth and clonogenicity of human colorectal cancer HT-29 cells. Apoptosis induced by morusin was characterized by accumulation of cells at the sub-G1phase, fragmentation of DNA, and condensation of chromatin. Morusin also inhibited the phosphorylation of IKK-α, IKK-β and IκB-α, increased expression of IκB-α, and suppressed nuclear translocation of NF-κB and its DNA binding activity. Dephosphorylation of NF-κB upstream regulators PI3K, Akt and PDK1 was also displayed. In addition, activation of caspase-8, change of mitochondrial membrane potential, release of cytochrome c and Smac/DIABLO, and activation of caspase-9 and -3 were observed at the early time point. Downregulation in the expression of Ku70 and XIAP was exhibited afterward. Caspase-8 or wide-ranging caspase inhibitor suppressed morusin-induced apoptosis. Therefore, the antitumor mechanism of morusin in HT-29 cells may be via activation of caspases and inhibition of NF-κB.
URI: http://rportal.lib.ntnu.edu.tw/handle/77345300/41470
ISSN: 0006-291X
Other Identifiers: ntnulib_tp_A0307_01_006
Appears in Collections:教師著作

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