Please use this identifier to cite or link to this item: http://rportal.lib.ntnu.edu.tw:80/handle/77345300/37061
Title: Opto-mechanical analysis for confocal laser scanning microscopes
Authors: 國立臺灣師範大學機電工程學系
Chang, Gao-Wei
Twu, Ming-Jenq
Lin, Yu-Hsuan
Liao, Chia-Cheng
Kuo, Hung-Zen
Issue Date: 2-Aug-2005
Publisher: The International Society for Optical Engineering (SPIE)
Abstract: The confocal imaging has become one of the most widely applied microscopic techniques in various fields, such as biotechnology, automation engineering, optical engineering, solid-state physics, metallurgy, integrated circuit inspection, etc. Confocal laser scanning microscopy (CLSM) is primarily based on the use of apertures in the detection path to provide the acquired three-dimensional images with satisfactory contrast and resolution. The major objective of this paper is to analyze the imaging performance of the confocal microscopes with varying opto-mechanical conditions. In this paper, the working principles of the one- and two-dimensional scanning mechanisms in the microscopic systems are first reviewed and verified by opto-mechanical simulations. Then, for the imaging performance, the tolerance to the fabrication and assembly of the optical components in conventional confocal microscopes is also investigated by simulations. The simulation results indicate the importance of eliminating the effects of stray light in the microscopic systems.
URI: http://rportal.lib.ntnu.edu.tw/handle/77345300/37061
Other Identifiers: ntnulib_tp_E0405_02_001
Appears in Collections:教師著作

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