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Title: Quantum-dot-embedded silica nanotubes as nanoprobes for simple and sensitive DNA detection
Authors: 國立臺灣師範大學化學系
Y.-H. Liu
Y.-Y. Tsai
H.-J. Chien
C.-Y Chen
Y.-F. Huang
J.-S. Chen
Y.-C. Wu
Chia-Chun Chen
Issue Date: 15-Apr-2011
Publisher: IOP Publishing
Abstract: We have developed a new technique using fluorescent silica nanotubes for simple and sensitive DNA detection. The quantum-dot-embedded silica nanotubes (QD-SNTs) were fabricated by a sol–gel reaction using anodic aluminum silica oxide (AAO) as a template. The fluorescent QD-SNTs of different colors were then immobilized with single-stranded DNA and used as nanoprobes for DNA detection. The optical and structural properties of QD-SNT nanoprobes were examined using photoluminescence spectroscopy, confocal microscopy and transmission electron microscopy (TEM). The QD-SNT nanoprobes were applied to detect dye-labeled target DNA in a solution phase. The obvious color change of the QD-SNT nanoprobes was observed visually under a simple microscope after the successful detection with target DNA. The quantitative analyses indicated that ~ 100 attomole of target DNA in one nanoprobe can generate a distinguishable and observable color change. The detection results also demonstrated that our assay exhibited high specificity, high selectivity and very low nonspecific adsorption. Our simple DNA assay based on QD-SNT nanoprobes is expected to be quite useful for the needs of fast DNA screening and detection applications.
ISSN: 0957-4484
Other Identifiers: ntnulib_tp_C0301_01_074
Appears in Collections:教師著作

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