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Titel: 探討1-Thio-β-D-glucose表面修飾螢光金奈米團簇於腦瘤細胞、大腸桿菌與線蟲代謝後之螢光變化
Studies of Fluorescence Changes of 1-Thio-β-D-glucose Modified Gold Nanoclusters in U-87 MG, E. coli and C. elegans
Autoren: 陳家俊
Chen, Chia-Chun
鐘冠蘭
Chung, Kuan-Lan
Stichwörter: 螢光金奈米團簇
表面修飾
1-Thio-β-D-glucose
螢光成像
生物感測
瓦氏效應
Fluorescent Gold Nanoclusters
Surface Modification
1-Thio-β-D-glucose
Fluorescence Imaging
Biosensor
Warburg Effect
Erscheinungsdatum: 2017
Zusammenfassung: 螢光金奈米團簇(Gold Nanoclusters, Au NCs)具有獨特的光學性質、易於進行表面修飾及良好的生物相容性等優點,使其具有生物顯影及生物感測之應用性。本研究利用穀胱甘肽做為還原劑製備出發橘紅色螢光之金奈米團簇,並以β-D硫代葡萄糖(1-Thio-β-D-glucose)修飾其表面,合成出表面修飾葡萄糖之螢光金奈米團簇(TGlu-Au NCs)。我們利用顯微注射及餵食的方式,使TGlu-Au NCs進入秀麗隱桿線蟲(C. elegans)體內,在線蟲的咽、體腔及肛門都能清楚看到TGlu-Au NCs的螢光成像。使用大腸桿菌(E. coli)觀察TGlu-Au NCs的螢光變化,得到大腸桿菌濃度上升會使TGlu-Au NCs螢光強度下降之結果,顯示TGlu-Au NCs做為生物感測器之潛力。另將腦瘤細胞之糖酵解酶取出與TGlu-Au NCs反應,發現TGlu-Au NCs的螢光強度隨著反應時間愈長而下降愈多,顯示腫瘤細胞發生糖解作用,證實TGlu-Au NCs可利用腦瘤細胞的瓦氏效應行標記之作用。
Fluorescent gold nanoclusters have been widely employed in the bioimaging and biosensing because of their unique optical properties, facile surface modification and high biocompatibility. In this study, we used L-Glutathione as a reductant to prepared fluorescent gold nanoclusters (Au NCs) and then the surface of Au NCs was modified with 1-Thio-β-D-glucose (TGlu-Au NCs). TGlu-Au NCs were respectively microinjected and fed into C. elegans to investigate their in vivo fluorescence imaging. The fluorescence of TGlu-Au NCs was easily visible in the lumen of the digestive system, extending from the pharynx to the anal region. Furthermore, the fluorescence changes of TGlu-Au NCs were studied with E. coli. TGlu-Au NCs at 0.4 (O.D.) showed higher decreasing rate of fluorescence intensity than that of TGlu-Au NCs at 0.27 (O.D.). In addition, the glycolytic enzyme of brain tumor cells (U-87 MG) was added into TGlu-Au NCs solution. After the reaction with the glycolytic enzyme, the fluorescence intensity of TGlu-Au NCs was decreased with the increase of time. The results confirmed the Warburg effect of TGlu-Au NCs in brain tumor cell.
URI: http://etds.lib.ntnu.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=%22http://etds.lib.ntnu.edu.tw/cgi-bin/gs32/gsweb.cgi?o=dstdcdr&s=id=%22G060442100S%22.&%22.id.&
http://rportal.lib.ntnu.edu.tw:80/handle/20.500.12235/100163
Sonstige Kennungen: G060442100S
Enthalten in den Sammlungen:學位論文

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