Browsing by Author "Chen, Chi-Chu"

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    Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay
    (2018-05-16) Chiu, Nan-Fu; Chen, Chi-Chu; Yang, Cheng-Du; Kao, Yu-Sheng; Wu, Wei-Ren
    Abstract In this study, we propose a modified gold nanoparticle-graphene oxide sheet (AuNP-GO) nanocomposite to detect two different interactions between proteins and hybrid nanocomposites for use in biomedical applications. GO sheets have high bioaffinity, which facilitates the attachment of biomolecules to carboxyl groups and has led to its use in the development of sensing mechanisms. When GO sheets are decorated with AuNPs, they introduce localized surface plasmon resonance (LSPR) in the resonance energy transfer of spectral changes. Our results suggest a promising future for AuNP-GO-based label-free immunoassays to detect disease biomarkers and rapidly diagnose infectious diseases. The results showed the detection of antiBSA in 10 ng/ml of hCG non-specific interfering protein with dynamic responses ranging from 1.45 nM to 145 fM, and a LOD of 145 fM. Considering the wide range of potential applications of GO sheets as a host material for a variety of nanoparticles, the approach developed here may be beneficial for the future integration of nanoparticles with GO nanosheets for blood sensing. The excellent anti-interference characteristics allow for the use of the biosensor in clinical analysis and point-of-care testing (POCT) diagnostics of rapid immunoassay products, and it may also be a potential tool for the measurement of biomarkers in human serum.
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    探討逐步調控氧化石墨烯之含氧官能基於電化學表面電漿子共振技術增強免疫感測晶片靈敏度之應用
    (2016) 陳吉助; Chen, Chi-Chu
    本研究利用電化學表面電漿子技術(Electorchemical surface plasmon resonanc, EC-SPR)進行氧化石墨烯(Graphene Oxide, GO)還原。透過SPR即時監測其還原過程，藉由偵測金屬-介電質之間表面折射率的變化與SPR角位移的改變，判斷氧化石墨烯的還原狀況。將電化學還原後的還原氧化石墨烯(Electrochemical Reduce Graphene Oxide, ERGO)進行阻抗分析，觀察其還原後的導電特性。並將此薄膜作為生物蛋白分子檢測薄膜，分析其檢測靈敏性。 本實驗採用兩種不同電化學還原技術進行GO的還原與比較，分別為循環伏安法(cyclic voltammetry, CV)與定電位還原法(Constant voltage)。在不同還原條件的ERGO薄膜，將使用電化學阻抗譜(Electrochemistry Impedance Spectroscopym, EIS)、X射線光電子能譜(X-ray photoelectron spectroscopy, XPS)以及傅立葉轉換紅外光譜(Fourier-Transform Infrared Spectrometer, FTIR)進行分析。 分析的結果顯示，利用電化學表面電漿子技術可以即時監測與逐步控制GO的碳氧比。還原後的ERGO薄膜，其氧官能基比例大幅的下降。使用恆定電位還原120秒之ERGO，其碳氧比由3.97增加至71.46，阻抗值也大比例下降，大幅度提升其導電性。 在生物感測方面，將牛血清蛋白(Bovine Serum Albumin, BSA)修飾於薄膜，並注入不同濃度的Anti-BSA進行阻抗分析，其偵測極限濃度可達到100 pg/ml的高靈敏性。而恆定電位還原法120秒的ERGO薄膜，其Anti-BSA的阻抗響應高於循環伏安法還原100圈的ERGO薄膜約兩倍。